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Cytiva NAP™ Columns, NAP-5
Use for rapid and efficient purification of DNA and oligonucleotides (>10-mer) in less than 15 minutes by gravity flow. Cytiva Life Sciences™ illustra™ NAP™ Columns are disposable columns prepacked with Sephadex™ G-25 DNA Grade
| Includes | Complete step-by-step protocol identifying the fixed volumes needed for equilibration, washing and sample elution |
|---|---|
| Product Type | NAP-5 |
| For Use With (Application) | Rapid and efficient purification of DNA and Oligonucleotides |
Cytiva Superdex™ 200 Increase 10/300 GL Prepacked Tricorn™ Columns
For size exclusion chromatography and analysis of proteins with molecular weights between 10,000 and 600,000. Cytiva Lifescience™ Superdex™ 200 Increase 10/300 GL Prepacked Tricorn™ Columns are designed for small scale preparative purification.
anti-Mouse IgG, peroxidase-linked species-specific whole antibody (from sheep) Secondary Antibody, Cytiva
Highly species-specific anti-Mouse IgG. Cytiva Lifescience™ anti-Mouse IgG, peroxidase-linked species-specific whole antibody (from sheep) Secondary Antibody is designed for applications such as protein blotting, ELISA and immunocytochemistry.
| Antigen | IgG, Whole Molecule |
|---|---|
| Purification Method | Affinity Purified |
| Host Species | Sheep |
| Conjugate | HRP |
| Classification | Monoclonal |
| Isotype | IgG |
| Primary or Secondary | Secondary |
Cytiva HisTrap™ FF Columns: 16 x 25mm
Use for optimized purification of histidine-tagged proteins directly from homogenized, unclarified cells by immobilized metal ion affinity chromatography (IMAC). Cytiva Life Sciences™ HisTrap™ FF Columns: 16 x 25mm is a ready-to-use column, prepacked with precharged Ni Sepharose™ 6 Fast Flow.
| Flow Rate | <20 mL/min. |
|---|---|
| Type | Pre-packed Column |
| Content And Storage | 4°C to 30°C, 20% Ethanol |
| Diameter (English) Inner | 0.6 in. |
| Description | HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC). |
| Max. Operating Pressure | 5 bar (0.5 MPa, 70 psi) |
| pH Range | 3 to 12 |
| Diameter (Metric) Inner | 16 mm |
| Material | Polypropylene |
| Support Type | 6% Cross-linked Agarose |
| Thickness (Metric) | 435 μm |
|---|---|
| Flow Rate | 47 sec./100mL |
| Particle Retention | 1.5 μm |
| Type | RTU Filter for Total Suspended Solids Analysis |
| Temperature (English) Max. | 1022°F |
| Description | Our Whatman glass microfiber filter papers in grade 934-AH RTU have been pre-washed, dried (or ignited), cooled, desiccated and weighed, which eliminates the pre-treating steps that are otherwise required by the US EPA Laboratory Standard Method 2540 part |
| Thickness (English) | 0.017 in. |
| Temperature (Metric) Max | 550°C |
| Quantity | 100 pieces |
| Grade | 934-AH RTU |
| Material | Borosilicate Glass |
| For Use With (Application) | Monitoring of rivers, lakes and coastal waters |
| Waterflow Rate | 341 mL/min |
Cytiva Whatman™ Mini-UniPrep™ Syringeless Filters with Preslit Septa
Processes sample loads in one-third the time. Whatman™ Mini-UniPrep™ Syringeless Filters with Preslit Septa provide a faster and easier way to remove particulates from samples being prepared for HPLC and UHPLC analysis.
| For Use With (Application) | Routine HPLC/UHPLC analysis; Composite assays; Content uniformity; Protein precipitation; Solubility testing; Dissolution testing; Sample filtration; Standard robotics on HPLC instruments with sensitive needles, allowing for higher throughput; Light sen |
|---|
Cytiva Whatman™ Binder-Free Glass Microfiber Filters, Grade GF/A
Combines high loading capacity with fine particle retention. Whatman™ Binder-Free Glass Microfiber Filters, Grade GF/A are ideal for gravimetric determination of airborne particulates, stack sampling and absorption methods of air pollution monitoring.
| Particle Retention | 1.6 μm |
|---|---|
| Temperature (English) Max. | 932°F |
| Shape | Circle |
| Description | GE’s Whatman Grade GF/A glass microfiber filters feature fine retention. GF/A filters are suitable for water quality parameter measurement and air filtration applications. |
| Temperature (Metric) Max | 500°C |
| Quantity | 100 Pieces |
| Grade | Grade GF/A |
| Material | Borosilicate Glass |
| For Use With (Application) | Used for high-efficiency general purpose laboratory filtration, including water pollution monitoring of effluents, for filtration of water, foodstuff analyses, protein filtration, and radioimmunoassay of weak β emitters. |
| Thickness (Metric) | 260 μm |
| Flow Rate | 13 sec./100mL |
| Type | Fine Retention Filter |
| Format | Disc |
| Thickness (English) | 0.01 in. |
| Waterflow Rate | 143 mL/min |
Cytiva Ficoll-Paque™ PLUS Media
Recognized standard in laboratories worldwide for isolation of human lymphocytes from peripheral blood. Cytiva™ Ficoll-Paque™ PLUS Media maintains the viability and a representative distribution of B and T lymphocytes.
| Content And Storage | 4–30°C |
|---|---|
| Form | Liquid |
| Product Type | Centrifugation Media |
| Color | Colorless to Slightly Yellow |
| Endotoxin Level | < 0.12 EU/mL |
| Shelf Life | 3 years |
| For Use With (Application) | Cell Isolation |
| Sterility | Sterile |
| Density | 1.078 g/mL |
Cytiva Percoll™ Centrifugation Media
For separation of cells, subcellular particles, and larger viruses (down to approx. 70S) under gentle conditions that preserve viability and morphological integrity. Cytiva™ Percoll™ Centrifugation Media Percoll is composed of colloidal silica coated with polyvinylpyrrolidone (PVP).
| Viscosity | 15cP |
|---|---|
| Content And Storage | 4–30°C |
| Form | Liquid |
| Product Type | Centrifugation Media |
| Color | Colorless to Slightly Yellow |
| pH | 8.5 to 9.5 |
| Shelf Life | 5 years |
| For Use With (Application) | Cell Isolation |
| Density | 1.135 g/mL |
| Osmolality | < 25 mOsm/kg |
Cytiva GFX™ PCR DNA and Gel Band Purification Kit
Purify and concentrate PCR products or DNA fragments ranging in size from 50 bp to 10 kb. Cytiva Life Sciences™ illustra™ GFX™ PCR DNA and Gel Band Purification Kit can be used to purify DNA from reaction volumes up to 100μL or agarose gel slices of up to 900mg.
| Includes | GFX™ columns, collection tubes, color-coded bottles of capture Buffer, Wash Buffer, two Elution Buffers |
|---|---|
| Description | Designed for the rapid purification and concentration of PCR products or DNA fragments ranging in size from 50 bp to 10 kb. This kit can be used to purify DNA from reaction volumes up to 100 μl or agarose gel slices up to 900 mg. |
| Product Type | illustra GFX PCR DNA and Gel Band Purification Kit |
| Formulation | Tris HCl and sterile Water |
| Sample Type | Agarose Gel Slices,DNA Solutions,Enzyme Reactions,PCR Mixtures |
| Sample Volume | 50 bp-10 kb |
| Yield | PCR 10 μl elution volume-65%, PCR 50 μl elution volume-82%, 300 mg agarose 10 μl elution volume-57%, 300 mg agarose 50 μl elution volume-91% |
| For Use With (Application) | Isolation and concentration of DNA fragments (50bp to 10kb) from PCR mixtures, DNA-containing Agarose Gel bands, Enzyme-based DNA modifications and restriction digestions |
Cytiva HisTrap™ HP Prepacked Columns
Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).
| Flow Rate | 4 mL/min. (1 mL), 5 mL/min. (5 mL) |
|---|---|
| Type | Pre-packed Column |
| Content And Storage | 4°C to 30°C |
| Description | HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery. |
| Max. Operating Pressure | 5 bar (0.5 MPa, 70 psi) |
| pH Range | 3 to 12 |
| Material | Polypropylene |
| Support Type | 6% Highly Cross-linked Agarose |
Cytiva Pharmalyte™ Carrier Ampholytes
Use in Isoelectric focusing. Cytiva Pharmalyte™ Carrier Ampholytes prepared by the co-polymerization of glycine, glycylglycine, amines, and epichlorohydrin, are available in five broad-range and four narrow-range pH intervals.
| Form | Liquid |
|---|---|
| Product Type | Pharmalyte carrier Ampholyte |
| For Use With (Equipment) | PhastSystem Automated Electrophoresis Unit |
| Formulation | Co-polymerization of Glycine, Glycylglycine, Amines and Epichlorohydrin |
| Color | Colorless to Light Yellow |
| Quantity | 25mL |
| Storage Requirements | 2° to 8°C |
| For Use With (Application) | IEF |
anti-Rabbit IgG, peroxidase-linked species-specific whole antibody (from donkey) Secondary Antibody, Cytiva
A highly species-specific anti-Rabbit IgG. Cytiva Lifescience™ anti-Rabbit IgG, peroxidase-linked species-specific whole antibody (from donkey) Secondary Antibody is used with Amersham™ ECL Western Blotting detection reagents.
| Antigen | IgG |
|---|---|
| Purification Method | Affinity Purified |
| Host Species | Donkey |
| Conjugate | HRP |
| Classification | Monoclonal |
| Isotype | IgG |
| Primary or Secondary | Secondary |