Thermo Scientific Western Blotting

Protein Detection and Analysis - Western Blotting

Maximize results and minimize hands-on time: Our all-encompassing range of solutions for western blotting analysis is meticulously designed and tested to meet the highest standards and minimize time and effort. Establish a robust foundation for your western blotting experiments.

Applications
Resources

Step 1: Gel Electrophoresis
Step 2: Transfer
Step 3: Blot Processing
Step 4: Detection
Step 5: Imaging and Analysis

Precast Mini and Midi Gels

Five distinct gel chemistries—enhance the separation of proteins with different molecular weights
WedgeWell—high loading capacity well options—the trusted performance of Invitrogen™ precast gels with twice the sample loading capacity. Also available in the high-throughput Midi gel format
Gel performance guarantee—meticulously tested for reliable, consistent performance

Invitrogen Mini Gel Tank System

Forward-facing well configuration—easier sample loading, monitor both gel runs simultaneously
Less running buffer required—two separate gel chambers, only use the buffer needed for each gel
Built to last—made of durable polycarbonate

Invitrogen Midi Gel Tank System

Higher throughput—run up to two midi gels and 52 samples at the same time
Separate chambers—use only the buffer needed for each gel
2-in-1 design—30 min, room temperature transfer in the same tank

Power Supplies

Bright LCD touch screen—easy to use and monitor run progress
Simple and intuitive Interface—enter and save custom protocols in few quick steps
Preprogrammed methods—start gel runs and transfers in seconds

Protein Ladders

Prestained multicolor—ready-to-load, for broad, high and low molecular weight proteins
Fluorescent optimized options—excellent for multiplexing experiments
Specialty options—IEF, native PAGE, and more

Protein Stains

Flexible—wide selection of in-gel and on-membrane protein stains
Comprehensive—including Coomassie dye-based, silver, fluorescent, and tagged fusion protein stains, as well as PTM protein gel stains
Compatible—MS, western blotting, and protein sequencing compatible stains available

iBlot 3 Dry Transfer System

Fast—transfer protein in as few as 3 minutes
Convenient—preprogrammed settings, preassembled transfer stacks, and minimal post-transfer cleanup
Reduced lab backlog—two independent transfer stations allow two users to simultaneously transfer using the same or different conditions

Power Blotter Semi-Dry Transfer System

Versatile—use with do-it-yourself, ready-to-build, or ready-to-use transfer stacks
Flexible to fit your lab’s needs—two transfer cassette options to support transfer of up to four mini or two midi gels at the same time
Integrated power supply—seamless operation between control unit and cassette, no external power supply required as with wet tank transfer

Wet Transfer Systems

Two-in-one tank systems—electrophoresis and transfer in the same tank
Conserve transfer buffer—only 220 mL for mini and 300 mL for midi gel transfers
Robust electrode plates—for highly efficient and reliable western transfers

Invitrogen iBind Automated Western Systems

Load and go—leverage sequential lateral flow technology with no batteries, shakers, trays, or timers required
Flexible throughput—pick a platform that matches your throughput needs; process one mini or midi blot, two mini blots, or six vertically cut strips using the same or different conditions
Reproducibility—help reduce variability introduced with more hands-on, traditional processing

Bandmate Automated Western Blot Processor

Simple setup—prepare block, wash, and antibody solutions let you load, program, and walk away
Your protocols, your buffers—no specialized reagents or protocols needed
Antibody recovery—recover antibody for reuse in future experiments, if desired

Primary Antibodies

Find your match—200,000 primary antibodies with broad species and application coverage
Extensive testing—advanced verification with demonstrated specificity and sensitivity
Enhanced reproducibility—take advantage of over 10,000 recombinant antibodies

Chemiluminescent Detection

If you need an everyday solution to analyze a broad range of protein expression levels, an exceptionally sensitive substrate, or a solution for quantitative western blotting, we have the right chemiluminescent substrate for you.

Fluorescent Detection

Reagents, buffers, and fluorescent conjugated antibodies for optimal fluorescent western blotting analysis

No-Stain Protein Labeling Reagents

Fast protein labeling—mix, label, and image gels or blots
Flexible visualization—UV or green fluorescent light
Total protein normalization—broad linear dynamic range (1–80 µg)

Secondary Antibodies for Western Blotting

Choice—wide variety of HRP-conjugated and Alexa Fluor™ secondary antibodies with a range of host and target species
Excellent multiplexing abilities—detect multiple proteins at the same time with the largest selection of Alexa Fluor dyes available
Higher signal-to-noise ratio—up to 5.8 times higher signal-to-noise ratio with Alexa Fluor Plus

Invitrogen iBright Imaging Systems

Smooth imaging experience with minimal effort—simple, clean touchscreen interface with logical workflows, complete with a suite of automated features
The core imaging capabilities that you need—fluorescent blot, chemiluminescent blot, protein gel, and DNA gel imaging
The special application capabilities that you want—image membrane stains, 2D strips, TLC plates, colorimetric blots, colony plates, perform total protein normalization and more

Resources

iWestern Brochure

The Invitrogen™ iWestern™ workflow features innovative, modern solutions designed to address processing efficiency, reproducibility, and robustness of results—because your time is precious.

Filename: 22341_iWestern Brochure_Thermo BID_Fisherised_Final.pdf
Size: 4 MB
Format: application/pdf