Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
- (1)
- (1)
- (123)
- (5)
- (63)
- (2)
- (1)
- (8)
- (2)
- (1)
- (10)
- (3)
- (2)
- (1)
- (4)
- (2)
- (9)
- (1)
- (2)
- (7)
- (126)
- (1)
- (130)
- (1)
- (1)
- (1)
- (8)
- (1)
- (4)
- (1)
- (10)
- (1)
- (1)
- (1)
- (28)
- (1)
- (5)
- (18)
- (1)
- (5)
- (2)
- (2)
- (1)
- (10)
- (1)
- (2)
- (52)
- (3)
- (7)
- (36)
- (180)
- (1)
- (1)
- (32)
- (2)
- (6)
- (3)
- (1)
- (2)
- (18)
- (1)
- (22)
- (1)
- (1)
- (5)
- (11)
- (7)
- (1)
- (79)
- (191)
- (12)
- (5)
- (6)
- (1)
- (5)
- (1)
- (1)
- (1)
- (1)
- (4)
- (117)
- (3)
- (3)
- (3)
- (7)
- (3)
- (5)
- (729)
- (5)
- (4)
- (4)
- (103)
- (30)
- (16)
- (28)
- (5)
- (7)
- (1)
- (4)
- (135)
- (7)
- (1)
- (6)
- (18)
- (40)
- (2)
- (39)
- (19)
- (26)
- (2)
- (2)
- (8)
- (6)
- (14)
- (21)
- (56)
- (8)
- (49)
- (8)
- (3)
- (15)
- (8)
- (4)
- (2)
- (3)
- (4)
- (7)
- (8)
- (68)
- (4)
- (2)
- (3)
- (5)
- (6)
- (7)
- (15)
- (3)
- (18)
- (4)
- (7)
- (12)
- (43)
- (2)
- (3)
- (1)
- (4)
- (2)
- (1)
- (3)
- (3)
- (3)
- (6)
- (3)
- (4)
- (1)
- (5)
- (6)
- (4)
- (1)
- (1)
- (2)
- (6)
- (1)
- (1)
- (12)
- (2)
- (3)
- (2)
- (2)
- (2)
- (3)
- (7)
- (1)
- (2)
- (4)
- (2)
- (2)
- (1)
- (3)
- (2)
- (3)
- (3)
- (1)
- (2)
- (1)
- (3)
- (3)
- (2)
- (3)
- (2)
- (1)
- (15)
- (4)
- (1)
- (1)
- (4)
- (3)
- (2)
- (2)
- (2)
- (1)
- (4)
- (2)
- (1)
- (5)
- (12)
- (2)
- (1)
- (1)
- (4)
- (22)
- (2)
- (43)
- (2)
- (1)
- (1)
- (3)
- (1)
- (9)
- (2)
- (1)
- (2)
- (7)
- (1)
- (5)
- (4)
- (1)
- (1)
- (54)
- (1)
- (3)
- (6)
- (3)
- (1)
- (15)
- (6)
- (1)
- (3)
- (1)
- (5)
- (23)
- (50)
- (15)
- (1)
- (60)
- (1)
- (16)
- (2)
- (3)
- (1)
- (13)
- (1)
- (51)
- (3)
- (9)
- (1)
- (2)
- (5)
- (1)
- (1)
- (1)
- (3)
- (1)
- (32)
- (6)
- (4)
- (2)
- (2)
- (29)
- (3)
- (1)
- (15)
- (4)
- (1)
- (6)
- (109)
- (1)
- (156)
- (2)
- (3)
- (6)
- (16)
- (50)
- (81)
- (1)
- (2)
- (38)
- (61)
- (1)
- (10)
- (3)
- (32)
- (1)
- (2)
- (17)
- (3)
- (4)
- (4)
- (2)
- (4)
- (21)
- (2)
- (7)
Filtered Search Results
Gibco™ PBS, pH 7.4
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”
Learn More About the Greener Choice Program
Gibco PBS, pH 7.4, is a balanced salt solution ideal for cell culture applications. It lacks calcium, magnesium, and phenol red, preventing cell adhesion and assay interference. Trusted for quality, Gibco PBS ensures reliable results in cell washing, transport, and reagent preparation.
| Physical Form | Liquid |
|---|---|
| pH | 7.3 to 7.5 |
| Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
| Chemical Name or Material | Phosphate Buffered Saline (PBS) |
| Recommended Storage | 15°C to 30°C |
| Concentration | 1 X |
| For Use With (Application) | Mammalian Cell Culture |
| Osmolality | 280 - 315 mOsm/kg |
| Sterility | Sterile-filtered |
Gibco™ DPBS, calcium, magnesium
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”
Learn More About the Greener Choice Program
DPBS, calcium, magnesium
| Includes | No Sodium Pyruvate |
|---|---|
| Physical Form | Liquid |
| pH | 7 to 7.2 |
| Chemical Name or Material | DPBS |
| Recommended Storage | 2° to 8°C |
| Concentration | 1 X |
| For Use With (Application) | Mammalian Cell Culture |
Gibco™ DPBS, no calcium, no magnesium
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”
Learn More About the Greener Choice Program
DPBS, no calcium, no magnesium
| Physical Form | Liquid |
|---|---|
| Chemical Name or Material | DPBS |
| Recommended Storage | 15°to 30°C |
| Concentration | 1 X |
Gibco™ PBS, pH 7.4
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”
Learn More About the Greener Choice Program
Gibco PBS, pH 7.4, is a balanced salt solution ideal for cell culture applications. It lacks calcium, magnesium, and phenol red, preventing cell adhesion and assay interference. Trusted for quality, Gibco PBS ensures reliable results in cell washing, transport, and reagent preparation.
| Includes | No Calcium, No Magnesium |
|---|---|
| Color | Clear |
| Packaging | Bottle |
| Physical Form | Liquid |
| pH | 7.4 |
| Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
| Chemical Name or Material | Phosphate Buffered Saline (PBS) |
| Concentration | 1 X |
| For Use With (Application) | Washing Cells Before Dissociation; Diluting Cells for Counting and Preparing Reagents; Transporting Cells or Tissue |
| Osmolality | 280 - 315 mOsm/kg |
| Sterility | Sterile-filtered |
| Solution Type | Phosphate Buffered Saline |
| Product Line | Gibco™ |
Gibco™ PBS (10X), pH 7.4
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”
Learn More About the Greener Choice Program
Phosphate-buffered saline (PBS) is a balanced salt solution that is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents.
| Includes | No Sodium Pyruvate |
|---|---|
| Color | White |
| Physical Form | Liquid |
| pH | 7.4 |
| Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
| Chemical Name or Material | Phosphate Buffered Saline (PBS) |
| Recommended Storage | Storage conditions: 15°C to 30°C Shipping conditions: Room temperature Shelf life: 24 months from date of manufacture |
| Concentration | 10 X |
| For Use With (Application) | Mammalian Cell Culture |
| Osmolality | 2800 - 3100 mOsm/kg |
| Sterility | Sterile-filtered |
| Solution Type | Phosphate Buffered Saline |
| Product Line | Gibco™ |
PBS Tablets, Phosphate Buffered Saline, Fisher BioReagents™
Phosphate Buffered Saline Tablets, CAS Number-7647-14-5, 7447-40-7, 100 tablets, Clear, Gray, White, 5/8 in., 600 SEC max., CAUTION!
| Physical Form | Liquid |
|---|---|
| Recommended Storage | Room temperature |
Gibco™ PBS (10X), pH 7.4
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”
Learn More About the Greener Choice Program
PBS (10X), pH 7.4
| Physical Form | Liquid |
|---|---|
| pH | 7.3 to 7.5 |
| Chemical Name or Material | Phosphate Buffered Saline (PBS) |
| Recommended Storage | 15°to 30°C |
| Concentration | 10 X |
| For Use With (Application) | Mammalian Cell Culture |
| Osmolality | 2800 to 3100 mOsm/kg |
| Color | White |
|---|---|
| Packaging | Poly Bottle |
| Physical Form | Powder |
| Recommended Storage | Room temperature |
Gibco™ DPBS (10X), calcium, magnesium
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”
Learn More About the Greener Choice Program
DPBS (10X), calcium, magnesium
| Includes | No Sodium Pyruvate |
|---|---|
| Physical Form | Liquid |
| pH | 4.3 to 4.7 |
| Chemical Name or Material | DPBS |
| Concentration | 10 X |
| For Use With (Application) | Mammalian Cell Culture |
| Osmolality | 2700 to 3200 mOsm/kg |
Phosphate Buffered Saline (PBS), 10X Powder, pH 7.4, Fisher BioReagents™
Phosphate Buffered Saline (PBS), 10X Powder provides a convenient way to make 1 L of 10X stock PBS solution.
Corning™ Phosphate Buffered Saline, 1X
SureTRACE
Supports traceability with guaranteed access to certificates and proactive change notifications.
Learn More
Supports traceability with guaranteed access to certificates and proactive change notifications.
Learn More
Used alone or in combination with other agents for washing tissues and cells.
| Packaging | Poly Cube |
|---|---|
| Physical Form | Liquid |
| pH | 7.3 to 7.5 |
| Recommended Storage | Room temperature |
| Melting Point | 0°C |
Gibco™ PBS, pH 7.2
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”
Learn More About the Greener Choice Program
PBS, pH 7.2
| Includes | No Sodium Pyruvate |
|---|---|
| Physical Form | Liquid |
| pH | 7.2 |
| Chemical Name or Material | Phosphate Buffered Saline (PBS) |
| Recommended Storage | 15°to 30°C |
| Concentration | 1 X |
| For Use With (Application) | Mammalian Cell Culture |
| Osmolality | 280 to 320 mOsm/kg |
Gibco™ PBS (10X), pH 7.2
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”
Learn More About the Greener Choice Program
PBS (10X), pH 7.2
| Physical Form | Liquid |
|---|---|
| pH | 7.1 to 7.3 |
| Chemical Name or Material | Phosphate Buffered Saline (PBS) |
| Recommended Storage | 15°to 30°C |
| Concentration | 10 X |
| For Use With (Application) | Mammalian Cell Culture |
| Osmolality | 2800 to 3200 mOsm/kg |