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PCR Supplies

A variety of products designed for use in various PCR procedures. Includes dNTPs, enzymes, assays, arrays, master mixes, reagents, and kits. Products can be used for standard, real-time, direct, high fidelity, hot start, and long range PCR protocols.
Real Time PCR Products (131)
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1 – 15 of 112 results
1

Applied Biosystems™ TaqMan™ Exogenous Internal Positive Control Reagents
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TaqMan™ Exogenous Internal Positive Control Reagents contain a pre-optimized internal positive control (IPC) with pre-designed primers and TaqMan™ probe.

EDGE
GC-Rich PCR Performance Low
Format Tube(s)
Internal Probe Modification TAMRA™ Quencher (3'), VIC™ (5')
Product Type Exogenous Internal Positive Control Reagent
Reaction Speed Standard
PCR Method qPCR
Product Line TaqMan
2
Promotions Available

Invitrogen™ Platinum™ Direct PCR Universal Master Mix
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Invitrogen Platinum Direct PCR Universal Master Mix is designed to amplify DNA directly from various samples without the need to purify DNA.

EDGE
Shipping Condition Dry Ice
Polymerase Platinum II Taq Hot-Start DNA Polymerase
GC-Rich PCR Performance High
Fidelity (vs. Taq) 1X
Size (Final Product) 8 kb
Reaction Format SuperMix or Master Mix
Product Type Direct PCR Universal Master Mix
Reaction Speed Fast
Hot Start Built-In Hot Start
Overhang 3'-A
Concentration 2X
For Use With (Application) Hot-start PCR,Real Time PCR (qPCR)
3
Promotions Available

Invitrogen™ TaqMan™ Cells-to-CT™ Express Kit
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The TaqMan Cells-to-CT Express Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification.

EDGE
Shipping Condition Frozen, Dry Ice
Target cDNA
Content And Storage Express Lysis Solution (room temperature or 4°C), Express ezDNase (-20°C), SuperScript IV VILO Master Mix (-20°C), SuperScript IV VILO No RT Control (-20°C), TaqMan Fast Advanced Master Mix (4°C)
Format Kit
Sample Type Mammalian Cells
Isolation Technology Direct cell lysis
Kit Type Direct Cell Lysis RT-qPCR
Purification Target RNA
Starting Material 10-100,000 Cells
For Use With (Application) Gene Expression
High-throughput Compatibility Yes
PCR Method 2-step RT-qPCR
Detection Method TaqMan
4
Promotions Available

Applied Biosystems™ AmpliTaq Gold™ 360 DNA Polymerase
GREENER_CHOICE

The Applied Biosystems AmpliTaq Gold 360 DNA Polymerase, when used with improved AmpliTaq Gold 360 Buffer, and the optional 360 GC Enhancer, amplifies a vast range of DNA sequence contexts.

EDGE
Shipping Condition Room Temp or Wet Ice
GC-Rich PCR Performance High
Fidelity (vs. Taq) 1X
Overhang 3'-A
Concentration 5 U/μL
For Use With (Application) Hot-start PCR
Exonuclease Activity 5' - 3'
Polymerase AmpliTaq Gold 360 DNA Polymerase
Format Tube
Size (Final Product) 5 kb or less
Reaction Format Separate Components
Product Type DNA Polymerase
Reaction Speed Standard
Hot Start Built-In Hot Start
Starting Material DNA
5

Applied Biosystems™ Eukaryotic 18S rRNA Endogenous Control (VIC™/MGB probe, primer limited)
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Eukaryotic 18S rRNA Endogenous Control (VIC™/MGB probe, primer limited)

EDGE
6
Promotions Available

Applied Biosystems™ Human GAPD (GAPDH) Endogenous Control (VIC™/MGB probe, primer limited)
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Human GAPD (GAPDH) Endogenous Control (VIC™/MGB probe, primer limited)

EDGE
7
Promotions Available

Invitrogen™ Cells-to-CT™ 1-Step TaqMan™ Kit
GREENER_CHOICE

Like other members of the Cells-to-CT™ product family, the Cells-to-CT™ 1-Step TaqMan™ Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.

EDGE
GC-Rich PCR Performance High
Format Tube
Product Type 1-Step qPCR kit
For Use With (Equipment) 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio™, ViiA™ 7 System, StepOnePlus™ System, StepOne™ System, Real-Time PCR Systems or Thermal Cyclers, 7500 Fast Dx System, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system
Sample Type Mammalian Cells
Reaction Speed 7 min.
Reagent Type Cell Lysis
Starting Material 10-100,000 Cells
For Use With (Application) Gene Expression
PCR Method 1-step RT-qPCR
Product Line Cells-to-CT, TaqMan
Detection Method TaqMan
8
Promotions Available

Invitrogen™ TaqMan™ Fast Advanced Cells-to-CT™ Kit
GREENER_CHOICE

The TaqMan Fast Advanced Cells-to-Ct Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification.

EDGE
Shipping Condition Dry Ice
GC-Rich PCR Performance High
Fidelity (vs. Taq) 2X
Concentration 2X
For Use With (Application) Gene Expression
High-throughput Compatibility High-throughput Compatible
PCR Method 2-step RT-qPCR
Detection Method TaqMan
Format Tube
Product Type Fast Advanced Cells-to-Ct Kit
Sample Type Mammalian cells
Reaction Speed 7 min.
Reagent Type Cell Lysis
Purification Target Lysate
Starting Material 10-100,000 Cells
Product Line Cells-to-CT
9
Promotions Available

Applied Biosystems™ AmpliTaq Gold™ 360 Master Mix
GREENER_CHOICE

AmpliTaq Gold 360 PCR Master Mix contains everything required for successful PCR amplification in one convenient package–all components are premixed and premeasured.

Shipping Condition Room Temp or Wet Ice
GC-Rich PCR Performance High
Fidelity (vs. Taq) 1X
Overhang 3'-A
Concentration 2X
For Use With (Application) Cloning,Hot-start PCR
Exonuclease Activity 5' - 3'
Polymerase AmpliTaq Gold 360 DNA Polymerase
Format Tube
Size (Final Product) 5 kb or less
Reaction Format SuperMix or Master Mix
Product Type PCR Master Mix
Reaction Speed Standard
Hot Start Built-In Hot Start
Starting Material DNA
10
Promotions Available

Applied Biosystems™ TaqMan™ Gene Expression Assay (FAM)
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Applied Biosystems TaqMan Gene Expression assays are used for quantitative real-time PCR analysis of gene expression and consist of a pair of unlabeled PCR primers and a TaqMan probe with a dye label (FAM) on the 5' end and a minor groove binder (MGB) and non-fluorescent quencher (NFQ) on the 3' end.

Shipping Condition Room Temperature
GC-Rich PCR Performance High
Content And Storage 1 tube containing 20X (XS, S, and M sizes) or 60X (L size) mix of pre-formulated assay (1 probe and 2 primers).

Store at -15°C to -25°C.
Fidelity (vs. Taq) 60 X
Product Type Gene Expression Assay
Reaction Speed Fast
PCR Method qPCR
Product Line TaqMan
Detection Method Primer-probe
11

Applied Biosystems™ TaqMan™ Gene Expression Assay, VIC
GREENER_CHOICE

Applied Biosystems TaqMan Gene Expression assays, VIC, are used for quantitative real-time PCR analysis of gene expression and consist of a pair of unlabeled PCR primers and a TaqMan probe with a dye label (VIC) on the 5' end and a minor groove binder (MGB) and nonfluorescent quencher (NFQ) on the 3' end.

GC-Rich PCR Performance High
Fidelity (vs. Taq) 60 X
Format Tube
Product Type Gene Expression Assay
For Use With (Equipment) 7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex, QuantStudio™ Absolute Q Digital PCR System
Reaction Speed Fast
PCR Method qPCR
Detection Method Primer-probe
12

Applied Biosystems™ Custom TaqMan™ Gene Expression Assay, FAM
GREENER_CHOICE

Applied Biosystems Custom TaqMan Gene Expression assays are the custom version of our predesigned TaqMan assays for quantitative real-time PCR.

Shipping Condition Room Temperature
Content And Storage 1 tube containing a 20X (S and M sizes) or 60X (L size) mix of pre-formulated assay (1 probe and 2 primers).

Store at -15 to -25°C.
Product Type Custom TaqMan Gene Expression
For Use With (Equipment) 7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex
Product Line TaqMan
13
Promotions Available

Applied Biosystems™ TaqMan™ Copy Number Reference Assay, human, RNase P
GREENER_CHOICE

Human TaqMan™ Copy Number Reference Assays are run with human TaqMan™ Copy Number Assays in a duplex real-time PCR reaction to detect and measure copy number variations (CNVs) and smaller regions in the human genome.

Shipping Condition Ambient Temperature
GC-Rich PCR Performance Low
Content And Storage Control Primer-Probe Set(s)
Purity or Quality Grade RNase-Free, DNase-Free
Internal Probe Modification TAMRA™ Quencher (3'), VIC™ (5')
Form Frozen
Gene Symbol RPPH1
Concentration 20X
PCR Method qPCR
Detection Method Primer-probe
Target RNase P
Purification Method HPLC
Format Tube
Product Type Configurable Assay
Reaction Speed Standard
5'Primer Modification None
Label or Dye VIC
3'Primer Modification None
Species Human
Product Line TaqMan
14
Promotions Available

Invitrogen™ Cells-to-CT™ 1-Step Power SYBR™ Green Kit
GREENER_CHOICE

Like other members of the Cells-to-CT™ product family, the Cells-to-CT™ 1-Step Power SYBR™ Green Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.

GC-Rich PCR Performance High
Format Tube
Product Type 1-Step qPCR kit
For Use With (Equipment) 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio™, ViiA™ 7 System, StepOnePlus™ System, StepOne™ System, Real-Time PCR Systems or Thermal Cyclers, 7500 Fast Dx System, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system
Sample Type Mammalian Cells
Reaction Speed 7 min.
Reagent Type Cell Lysis
Starting Material 10-100,000 Cells
For Use With (Application) Gene Expression
PCR Method 1-step RT-qPCR
Product Line Cells-to-CT™, Power SYBR™ Green
Detection Method SYBR
15
Promotions Available

Applied Biosystems™ TaqMan™ MicroRNA Assay
GREENER_CHOICE

Applied Biosystems TaqMan MicroRNA Assays quantitate miRNAs with the specificity and sensitivity of TaqMan Assay chemistry. A simple, two-step protocol requires only reverse transcription with an miRNA-specific primer, followed by real-time PCR with TaqMan probes.

Shipping Condition Room Temperature
GC-Rich PCR Performance High
Content And Storage • 1 tube containing RT primer at concentration of 5X (XS and S sizes), 20X (M size), or 60X (L size)
• 1 tube containing a 20X (XS, S, and M sizes) or 60X (L size) mix of pre-formulated assay (1 probe and 2 primers).

Store at -15 to -25°C.
Format Tube
For Use With (Equipment) 7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex, QuantStudio™ Absolute Q Digital PCR System
Reaction Speed Fast
PCR Method qPCR
Product Line TaqMan