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Real Time PCR Assays and Arrays

Array cards and plates, endogenous controls, kits, and other PCR products designed to be used while performing quantitative real-time PCR assays. Ideal for various applications including gene-expression research and diagnostic testing.
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Promotions Available

Applied Biosystems™ TaqMan™ Gene Expression Assays

Applied Biosystems TaqMan Gene Expression assays are used for quantitative real-time PCR or digital PCR analysis of gene expression and consist of a pair of unlabeled PCR primers and a TaqMan probe with a dye label (FAM) on the 5' end and a minor groove binder (MGB) and non-fluorescent quencher (NFQ) on the 3' end.

Shipping Condition Room Temperature
GC-Rich PCR Performance High
Fidelity (vs. Taq) 60 X
Product Type Gene Expression Assay
Reaction Speed Fast
PCR Method qPCR
Product Line TaqMan
Detection Method Primer-probe
2
Promotions Available

Applied Biosystems™ TaqMan™ RNase P Detection Reagents Kit

TaqMan™ RNase P Detection Reagents Kit

EDGE
3
Promotions Available

Applied Biosystems™ TaqMan™ SNP Genotyping Assays

5' nuclease assay to discriminate between two alleles of a specific SNP for use in genotyping studies.

For Use With (Application) Genotyping
4
Promotions Available

Applied Biosystems™ Single-Tube TaqMan™ SNP Genotyping Assays

5' nuclease assay to discriminate between two alleles of a specific SNP for use in genotyping studies

5
Promotions Available

Applied Biosystems™ Single-Tube TaqMan™ SNP Genotyping Assays

5' nuclease assay to discriminate between two alleles of a specific SNP for use in genotyping studies

6
Promotions Available

Applied Biosystems™ Single-Tube TaqMan™ SNP Genotyping Assays

5' nuclease assay to discriminate between two alleles of a specific SNP for use in genotyping studies

7

Applied Biosystems™ TaqMan™ MicroRNA Assay

Applied Biosystems TaqMan MicroRNA Assays quantitate miRNAs with the specificity and sensitivity of TaqMan Assay chemistry. A simple, two-step protocol requires only reverse transcription with an miRNA-specific primer, followed by real-time PCR with TaqMan probes.

Shipping Condition Room Temperature
GC-Rich PCR Performance High
Content And Storage • 1 tube containing RT primer at concentration of 5X (XS and S sizes), 20X (M size), or 60X (L size)
• 1 tube containing a 20X (XS, S, and M sizes) or 60X (L size) mix of pre-formulated assay (1 probe and 2 primers).

Store at -15 to -25°C.
Format Tube
For Use With (Equipment) 7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex, QuantStudio™ Absolute Q Digital PCR System
Reaction Speed Fast
PCR Method qPCR
Product Line TaqMan
8
Promotions Available

Applied Biosystems™ TaqMan™ MGB Probe

Custom TaqMan probes are dual-labeled primers suitable for use in any real-time PCR application. They incorporate a 5' reporter dye and a 3' nonfluorescent quencher/minor groove binder (MGB). Choose among FAM, VIC, ABY, JUN, Cy5, TET, and NED as reporters.

Purification Method HPLC
Purity or Quality Grade HPLC
Format Tube
Internal Probe Modification None
For Use With (Equipment) 7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex, QuantStudio™ Absolute Q Digital PCR System
Form Liquid
3'Primer Modification MGB-NFQ
For Use With (Application) Real Time PCR (qPCR)
Product Line TaqMan
9
Promotions Available

Applied Biosystems™ TaqMan™ TAMRA Probe

Custom TaqMan™ Probe

Shipping Condition Room Temperature
Purification Method HPLC
Content And Storage 1 tube containing a dual-labeled probe in solution or dried down.

Store at -15°C to -25°C
Purity or Quality Grade HPLC
Format Tube
Internal Probe Modification None
Product Type TAMRA Probe
For Use With (Equipment) 7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex
Form Liquid or Dried Down
5'Primer Modification FAM, TET, VIC
3'Primer Modification TAMRA
For Use With (Application) Real Time PCR (qPCR)
Product Line TaqMan
10
Promotions Available

Applied Biosystems™ TaqMan™ Gene Expression Assay, VIC

Applied Biosystems TaqMan Gene Expression assays, VIC, are used for quantitative real-time PCR or digital PCR analysis of gene expression and consist of a pair of unlabeled PCR primers and a TaqMan probe with a dye label (VIC) on the 5' end and a minor groove binder (MGB) and nonfluorescent quencher (NFQ) on the 3' end.

GC-Rich PCR Performance High
Fidelity (vs. Taq) 60 X
Format Tube
Product Type Gene Expression Assay
For Use With (Equipment) 7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex, QuantStudio™ Absolute Q Digital PCR System
Reaction Speed Fast
PCR Method qPCR
Detection Method Primer-probe
11

Applied Biosystems™ TaqMan™ Pri-miRNA Assay

TaqMan Pri-miRNA Assays quantitate primary microRNA (pri-miRNA) transcripts, the most upstream RNA molecules in the microRNA biogenesis pathway.

Shipping Condition Room Temperature
GC-Rich PCR Performance High
Content And Storage 1 tube containing a 20X (S and M sizes) or 60X (L size) mix of pre-formulated assay (1 probe and 2 primers).

Store at -15 to -25°C.
Fidelity (vs. Taq) 60 X
Format Tube
Product Type Pri-miRNA Assay
Reaction Speed Fast
PCR Method qPCR
Product Line TaqMan
12
Promotions Available

Applied Biosystems™ TaqMan™ Copy Number Assay

Applied Biosystems TaqMan Copy Number Assays use gold-standard TaqMan MGB probe chemistry to evaluate the copy number of genomic DNA targets using Applied Biosystems real-time PCR instruments and software.

Shipping Condition Room Temperature
Content And Storage 1 tube containing a 20X (S and M sizes) or 60X (L size) mix of pre-formulated assay (1 probe and 2 primers).

Store at -15 to -25°C.
Purity or Quality Grade RNase-Free, DNase-Free
Internal Probe Modification FAM (5'), MGB (Minor Groove Binder) (3')
Form Frozen
PCR Method qPCR
Detection Method Primer-probe
Purification Method Solid Phase Extraction (SPE)
Product Type Copy Number Assay
For Use With (Equipment) 7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex, QuantStudio™ Absolute Q Digital PCR System
Reaction Speed Fast
5'Primer Modification None
Label or Dye FAM
3'Primer Modification None
Species Human
Product Line TaqMan
13

Applied Biosystems™ TaqMan™ SCID/SMA Assay

A multiplex real-time PCR assay that detects the SMN1 gene and TREC, as well as the RNase P gene as an internal genomic control.

Shipping Condition Dry Ice
Content And Storage • DNA Extract All Reagents Kit
• TaqPath ProAmp Multiplex Master Mix
• TaqMan SCID/SMA Assay

Upon receipt, store at -20°C. After opening, store at 4°C.
For Use With (Equipment) QuantStudio™ 12 k Flex, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, QuantStudio™ Dx System (in RUO Mode)
Sample Type DNA (Genomic), cDNA
Product Line TaqMan
Detection Method Primer-probe
14
Promotions Available

Applied Biosystems™ Custom TaqMan™ Copy Number Assay

Applied Biosystems TaqMan Copy Number Assays use gold-standard TaqMan MGB probe chemistry to evaluate the copy number of genomic DNA targets using Applied Biosystems real-time PCR instruments and software.

Shipping Condition Room Temperature
15

TaqMan™ SCID/SMA Plus Assay

A multiplex real-time PCR assay that detects the SMN1 gene, TREC, and KREC, as well as the RNase P gene as an internal genomic control.

Shipping Condition Dry Ice
Content And Storage • DNA Extract All Reagents Kit
• TaqPath ProAmp Multiplex Master Mix
• TaqMan SCID/SMA Plus Assay

Upon receipt, store at -20°C. After opening, store at 4°C.
For Use With (Equipment) QuantStudio™ 12 k Flex, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, QuantStudio™ Dx System (in RUO Mode)
Product Line TaqMan
Detection Method Primer-probe