Crosslinking, Labeling and Protein Modification
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Cytiva Amersham™ CyDye™ Post-Labeling Reactive Dye Packs
Reactive CyDye fluors specifically optimized for nucleic acid microarray labeling. Cytiva CyDye Post-Labelling Reactive Dye Pack provides conveniently packaged, highly reactive Cy3 and Cy5 monofunctional NHS ester to label probes via the post-labeling (amino allyl) route.
Cytiva Cy3 NHS Ester Mono-Reactive CyDye
Use for highly accurate labeling of amine groups. Cytiva Cy3 NHS Ester Mono-Reactive CyDye contain derivitized CyDyes that carry only one reactive group on each dye molecule for accurate labeling of amine groups, for example on antibodies, peptides, or oligonucleotides.
| Kit Contents | Foil packs of pre-measured dried dye |
|---|---|
| Format | Dried |
| pH Range | 3 to 10 |
| Product Type | Cy3 NHS Ester |
| Color | Red |
| Label or Dye | Cy3 |
Cytiva DeStreak Rehydration Solution
Highly suitable for both analytical and preparative gels. DeStreak Rehydration Solution improves reproducibility and quality of 2-D gels by preventing streaking.
| Content And Storage | 2°C to 8°C |
|---|---|
| Kit Contents | Optimized concentrations of urea, thiourea, CHAPS and Destreak reagent |
| pH Range | >7 |
| Product Type | Destreak Reagent |
| For Use With (Application) | Improves reproducibility and quality of 2D Gels by preventing streaking |
Cytiva Amersham™ CyDye™ Value Packs (bis-Reactive NHS Ester)
Suitable for general labeling of amine residues
| Kit Contents | Foil packs, each containing 5, 10 or 50mg dried dye |
|---|
Cytiva Cy5.5 NHS Ester Mono-Reactive CyDye
Use for highly accurate labeling of amine groups. Cytiva Cy5.5 NHS Ester Mono-Reactive CyDye contain derivitized CyDyes that carry only one reactive group on each dye molecule for accurate labeling of amine groups, for example on antibodies, peptides, or oligonucleotides.
| Kit Contents | Foil packs of premeasured dye |
|---|---|
| Format | Powdered |
| pH Range | 3 to 10 |
| Product Type | Cy5.5 NHS Ester |
| Color | Blue |
| Label or Dye | Cy5.5 |
Cytiva Cy™3B Mono-reactive NHS Ester Fluorescent Dyes
Combines exceptional photostability with bright and intense signals. Cytiva Cy™3B NHS Ester Mono-Reactive CyDye™ provides targeted and specific labeling of amine residues on antibodies, peptides, or oligonucleotides in fluorescence polarization applications.
| Format | Solid |
|---|---|
| Product Type | Cy3B NHS Ester |
| Molecular Weight (g/mol) | 771 |
| Formulation | 100% Cy3B (NHS ester) |
| Color | Red |
| Solubility | Soluble in aqueous buffers |
| Emission | 570 |
| Purity | >99% |
| Excitation | 559 |
Cytiva Amersham™ CyDye™ Value Packs (mono-Reactive Maleimide)
For the selective labeling of free sulfhydryl groups such as cysteine residues in peptides, proteins, and oligonucleotides
| Kit Contents | Foil packs, each containing 1, 5 or 25mg dried dye |
|---|---|
| pH Range | 3 to 10 |
Cytiva Amersham™ CyDye™ Value Packs (mono-Reactive NHS Ester)
Use for highly accurate labeling of amine groups. Cytiva Amersham™ CyDye™ Value Packs (mono-Reactive NHS Ester) contain derivitized CyDyes that carry only one reactive group on each dye molecule for accurate labeling of amine groups, for example on antibodies, peptides, or oligonucleotides.
| Kit Contents | Foil packs, each containing 1, 5, 10, 25 or 50mg dried dye |
|---|---|
| pH Range | 3 to 10 |
Cytiva Amersham™ Cy™3, Cy™ 5 Maleimide mono-Reactive Dye 5-packs
More selective method of antibody labeling that reduces the possibility of the label interfering with subsequent antibody-antigen reactions. Amersham™ Cy™3, Cy™ 5 Maleimide mono-Reactive Dye packs are packaged in premeasured amounts and foil-sealed to ensure consistent labelings.
| Content And Storage | 2°C to 8°C, protected from light. Reconstituted material may be stored for up to 2 weeks at -20°C in aliquots to avoid repeat freeze thaw cycles. |
|---|---|
| Kit Contents | Five vials of dye |
| Solubility | Soluble in cold water and hot water |
| Sufficient For | Labeling 1mg Protein |
| Quantity | 5 vials each sufficient to label 1 mg protein |
Cytiva CyDye™ DIGE Fluors: Cy5
Allow detection of up to three prelabeled protein samples and standards on the same 2-D electrophoresis gel
| Content And Storage | -15°C to -30°C |
|---|---|
| Format | Solid |
| Product Type | Amersham CyDye DIGE Fluor Cy5 Minimal Dye |
| Color | Red |
| For Use With (Application) | For general 2-D applications where sufficient amounts of sample are available, detection of up to three prelabeled protein samples and standards on the same 2-D electrophoresis gel |
Cytiva CyDye™ DIGE Fluors: Cy3
Allow detection of up to three prelabeled protein samples and standards on the same 2-D electrophoresis gel
| Content And Storage | -15°C to -30°C |
|---|---|
| Format | Solid |
| Product Type | Amersham CyDye DIGE Fluor Cy3 Minimal Dye |
| Color | Green |
| For Use With (Application) | For general 2-D applications where sufficient amounts of sample are available, detection of up to three prelabeled protein samples and standards on the same 2-D electrophoresis gel |
Cytiva CyDye™ DIGE Fluors: Cy2
Allow detection of up to three prelabeled protein samples and standards on the same 2-D electrophoresis gel
| Content And Storage | -15°C to -30°C |
|---|---|
| Format | Solid |
| Product Type | Amersham CyDye DIGE Fluor Cy2 Minimal Dye |
| Color | Blue |
| For Use With (Application) | For general 2-D applications where sufficient amounts of sample are available, detection of up to three prelabeled protein samples and standards on the same 2-D electrophoresis gel |
Cytiva Amersham™ Streptavidin-Fluor Conjugates
Use for in situ hybridization and flow cytometry. Cytiva Life Sciences™ Amersham™ Streptavidin-Fluor Conjugates consist of streptavidin conjugated to a range of fluorescent dyes.
| Formulation | This product is supplied as a lyophilized solid in Phosphate-buffered Saline (0.02M Potassium Phosphate, 0.15M NaCl) pH 7.2, containing 1%(w/v) Bovine Serum Albumin and 0.1% (w/v) Sodium Azide, reconstitute with 1mL of deionized Water, dispense into suitable aliquots and freeze, avoid repeated freeze-thaw cycles |
|---|---|
| Quantity | 1 mg |
| For Use With (Application) | Suitable for use in the Fluorescent analysis of Biotinylated samples |
Cytiva CyDye™ DIGE Fluor Minimal Dye Labeling Kits
Allow detection of up to three prelabeled protein samples and standards on the same 2-D electrophoresis gel. Amersham CyDye™ DIGE Fluor Minimal Labeling Kits contain size- and charge-matched fluorescent dyes specifically designed for detecting protein abundance differences in 2-D Fluorescence Difference Gel Electrophoresis using Ettan DIGE system.
| Content And Storage | -15°C to -30°C |
|---|---|
| Format | Solid |
| Color | Blue, Green, Red |
Cytiva CyDye™ DIFluors: Saturation Dye Labeling Kits
Allow detection of up to three prelabeled protein samples and standards on the same 2-D electrophoresis gel
| Content And Storage | -15°C to -30°C |
|---|---|
| Format | Solid |
| Product Type | Amersham CyDye DIGE Fluor Labeling Kit |
| Color | Green, Red |