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Real Time PCR Products

A range of products specifically designed for real-time PCR workflows, a process that monitors the amplification of a targeted DNA molecule during the PCR reaction, not at its end, as in conventional PCR. Products include assays, reagents, kits, and master mixes.
Real Time PCR Master Mixes (42)
Real Time PCR Reagents and Kits (8)

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1 – 15 of 21 results
1

Thermo Scientific™ Luminaris Color HiGreen qPCR Master Mix

Thermo Scientific Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR.

EDGE
Polymerase Taq DNA Polymerase
GC-Rich PCR Performance High
Fidelity (vs. Taq) 2 X
Product Type qPCR Master Mix
For Use With (Equipment) BioRad CFX96™, MJ Chromo4, MJ Opticon, Eppendorf MasterCycler RealPlex, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, Corbett RotorGene
Sample Type DNA, dsDNA
Reaction Speed Standard
Concentration 2X
For Use With (Application) Gene Expression
PCR Method qPCR
Product Line Luminaris
Detection Method SYBR
2

Thermo Scientific™ Maxima SYBR Green qPCR Master Mix (2X), with separate ROX vial

Ready-to-use solution optimized for qPCR and 2-step RT-qPCR.

EDGE
Polymerase Taq DNA Polymerase
GC-Rich PCR Performance High
Format Tube
Product Type Real Time PCR SYBR Master Mix
For Use With (Equipment) BioRad CFX96™, MJ Chromo4, MJ Opticon, Eppendorf MasterCycler RealPlex, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, Corbett RotorGene
Sample Type DNA, dsDNA
Reaction Speed Standard
Concentration 2X
For Use With (Application) Gene Expression
PCR Method qPCR
Product Line Maxima
Detection Method SYBR
3

Thermo Scientific™ Maxima Probe qPCR Master Mix (2X), with separate ROX vial

Optimize qPCR with probe chemistry using standard cycling protocols with these ready-to-use qPCR master mixes.

EDGE
Polymerase Taq DNA Polymerase
GC-Rich PCR Performance High
Format Tube
Product Type qPCR Master Mix
For Use With (Equipment) BioRad CFX96™, BioRad iCycler iQ, BioRad iQ5, BioRad MyiQ, MJ Chromo4, Eppendorf MasterCycler RealPlex, Corbett RotorGene, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, MJ Opticon
Sample Type DNA, dsDNA
Reaction Speed Standard
Concentration 2X
For Use With (Application) Gene Expression
PCR Method qPCR
Product Line Maxima
Detection Method Primer-probe
4

Thermo Scientific™ DyNAmo Flash SYBR Green qPCR Kit

Developed for fast real-time qPCR, the kit provides qPCR results faster than most SYBR Green kits without compromising the qPCR performance.

EDGE
Polymerase Tbr DNA polymerase
GC-Rich PCR Performance High
Format Tube
Product Type Real Time PCR SYBR Master Mix
For Use With (Equipment) 7000 System, 7300 System, 7500 Fast System, 7500 System, 7700 System, 7900HT Fast System, 7900HT System, BioRad CFX96™, QuantStudio™, ViiA™ 7 System, Bio-Rad CFX384 Touch™, Roche LightCycler 480, 5700 Sequence Detection System, StepOnePlus™ System, Eppendorf MasterCycler RealPlex, Corbett RotorGene, StepOne™ System, Stratagene Mx4000, Stratagene Mx3005P, Stratagene Mx3000P, MJ Opticon, MJ Chromo4, Cepheid SmartCycler
Sample Type DNA, dsDNA
Reaction Speed Fast
Kit Type DyNAmo Flash SYBR Green qPCR Kit
Concentration 2X (qPCR Mix), 50X (Dye)
For Use With (Application) Gene Expression
PCR Method qPCR
Product Line DyNAmo Flash
Detection Method SYBR
5

Thermo Scientific™ ABsolute Blue QPCR Mix, with separate ROX vial

ABsolute Blue qPCR Mixes utilize Thermo-Start Taq hot start enzyme for increased sensitivity.

Polymerase Taq DNA Polymerase
GC-Rich PCR Performance High
Fidelity (vs. Taq) 2 X
Format Tube
Product Type qPCR Mix
For Use With (Equipment) BioRad CFX96™, BioRad iCycler iQ, BioRad iQ5, BioRad MyiQ, MJ Chromo4, Eppendorf MasterCycler RealPlex, Corbett RotorGene, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, MJ Opticon
Sample Type DNA, dsDNA
Reaction Speed Standard
Concentration 2X
PCR Method qPCR
Product Line ABsolute Blue
Detection Method Primer-probe
6

Thermo Scientific™ Verso 1-step RT-qPCR Kit, SYBR Green, with separate ROX vial

The Verso RT enzyme delivers high processivity and can be used at temperatures up to 57°C, delivering effective transcription through difficult RNA secondary structures.

GC-Rich PCR Performance High
Format Tube
Product Type 1-Step RT-qPCR Kit
For Use With (Equipment) BioRad CFX96™, MJ Chromo4, MJ Opticon, Eppendorf MasterCycler RealPlex, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, Corbett RotorGene
Sample Type RNA
For Use With (Application) Gene Expression,Pathogen Detection
PCR Method 1-step RT-qPCR
Product Line Verso
Detection Method SYBR
7

Thermo Scientific™ ABsolute QPCR Mix, SYBR Green, no ROX

Optimized for SYBR Green chemistry and contains all the components necessary to perform quantitative PCR, with the exception of template and primers.

Polymerase Taq DNA Polymerase
GC-Rich PCR Performance High
Product Type Real Time PCR SYBR Master Mix
For Use With (Equipment) BioRad CFX96™, MJ Chromo4, MJ Opticon, Eppendorf MasterCycler RealPlex, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, Corbett RotorGene
Sample Type DNA, dsDNA
Reaction Speed Standard
Concentration 2X
Compatibility qPCR Cyclers
For Use With (Application) Gene Expression
PCR Method qPCR
Product Line ABsolute
Detection Method SYBR
8

Thermo Scientific™ ABsolute QPCR Mix, no ROX

Thermo Scientific ABsolute qPCR Mixes are optimized for use with hybridization probes and contain all the components necessary to perform quantitative PCR, with the exception of template and primers/probes.

Polymerase Taq DNA Polymerase
For Use With (Equipment) BioRad CFX96™, BioRad iCycler iQ, BioRad iQ5, BioRad MyiQ, MJ Chromo4, Eppendorf MasterCycler RealPlex, Corbett RotorGene, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, MJ Opticon
Sample Type DNA, dsDNA
Reaction Speed Standard
PCR Method qPCR
Product Line ABsolute
Detection Method Primer-probe
9

Thermo Scientific™ DyNAmo HS SYBR Green qPCR Kit

Contains a modified Thermus brockianus DNA polymerase and all the other reagents needed for qPCR.

Polymerase Tbr DNA polymerase
GC-Rich PCR Performance High
Format Tube
Product Type Real Time PCR SYBR Master Mix
For Use With (Equipment) 7000 System, 7300 System, 7500 Fast System, 7500 System, 7700 System, 7900HT Fast System, 7900HT System, BioRad CFX96™, QuantStudio™, ViiA™ 7 System, Bio-Rad CFX384 Touch™, Roche LightCycler 480, 5700 Sequence Detection System, StepOnePlus™ System, Eppendorf MasterCycler RealPlex, Corbett RotorGene, StepOne™ System, Stratagene Mx4000, Stratagene Mx3005P, Stratagene Mx3000P, MJ Opticon, MJ Chromo4, Cepheid SmartCycler
Sample Type DNA, dsDNA
Reaction Speed Standard
Kit Type DyNAmo HS SYBR Green qPCR Kit
Concentration 2X (qPCR Mix), 50X (Dye)
For Use With (Application) Gene Expression
PCR Method qPCR
Product Line DyNAmo
Detection Method SYBR
10

Thermo Scientific™ Verso 1-step RT-qPCR Kit, with separate ROX vial

The Verso RT enzyme delivers high processivity and can be used at temperatures up to 57°C, delivering effective transcription through difficult RNA secondary structures.

GC-Rich PCR Performance High
Format Tube
Product Type 1-Step RT-qPCR Kit
For Use With (Equipment) BioRad CFX96™, BioRad iCycler iQ, BioRad iQ5, BioRad MyiQ, MJ Chromo4, Eppendorf MasterCycler RealPlex, Corbett RotorGene, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, MJ Opticon
Sample Type RNA
For Use With (Application) Gene Expression,Pathogen Detection
PCR Method 1-step RT-qPCR
Product Line Verso
Detection Method Primer-probe
11

Invitrogen™ EXPRESS One-Step Superscript™ qRT-PCR Kit, with premixed ROX

Next-generation, real-time qRT-PCR reagents designed to complement fast-cycling instruments. Get the turnaround time you want with the data quality you need.

Polymerase Taq DNA Polymerase
Content And Storage EXPRESS One-Step SuperScript™ qRT-PCR SuperMixes are composed of 2 modules: an EXPRESS RT module (containing SuperScript™ III Reverse Transcriptase and RNAseOUT™) and an EXPRESS qPCR SuperMix module with ROX premixed or in a separate tube.

Store at -20°C upon receipt. Once thawed, the EXPRESS qPCR SuperMix can be stored for up to 1 month at +4·C. Product is guaranteed stable for 6 months when properly stored.
Format Tube
Product Type qRT-PCR Kit
For Use With (Equipment) 7000 System, 7300 System, 7700 System, 7900HT Fast System, 7900HT System, Eppendorf™ MasterCycler™ RealPlex, StepOne™, Fast Mode, StepOne™, Standard Mode
Sample Type RNA
Reaction Speed Fast or Standard
For Use With (Application) Gene Expression
PCR Method 1-step RT-qPCR
Product Line SuperScript™
Detection Method Primer-probe
12

Thermo Scientific™ ABsolute Blue QPCR Mix, SYBR Green, with separate ROX vial

Incorporates an inert blue dye to significantly enhance the contrast between reagent and plastic, making verification of master mix dispensing quick, easy, and foolproof.

Polymerase Taq DNA Polymerase
GC-Rich PCR Performance High
Format Tube
Product Type Real Time PCR SYBR Master Mix
For Use With (Equipment) BioRad CFX96™, MJ Chromo4, MJ Opticon, Eppendorf MasterCycler RealPlex, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, Corbett RotorGene
Sample Type DNA, dsDNA
Reaction Speed Standard
Concentration 2X
Compatibility Block-Based qPCR Instruments
For Use With (Application) Gene Expression,Genotyping,siRNA Validation
PCR Method qPCR
Product Line ABsolute Blue
Detection Method SYBR
13

Invitrogen™ EXPRESS qPCR Supermix, with premixed ROX

EXPRESS qPCR SuperMix kits are compatible with fast-cycling instruments for your probe-based qPCR assays.

GC-Rich PCR Performance High
Content And Storage EXPRESS qPCR SuperMix kits are provided as a 2X qPCR SuperMix with ROX premixed or in a separate tube.

Store at -20°C upon receipt. Once thawed, the EXPRESS qPCR SuperMix can be stored for up to 1 month at +4·C. Product is guaranteedstable for 6 months when properly stored.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
Format Tube
Product Type Express qPCR Supermix
For Use With (Equipment) 7000 System, 7300 System, 7700 System, 7900HT Fast System, 7900HT System, Eppendorf™ MasterCycler™ RealPlex, StepOne™, Fast Mode, StepOne™, Standard Mode
Sample Type DNA (Genomic), cDNA
Reaction Speed Fast or Standard
For Use With (Application) Gene Expression
PCR Method qPCR
Detection Method Primer-probe
14

Thermo Scientific™ Luminaris HiGreen qPCR Master Mix

Thermo Scientific Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR.

Polymerase Taq DNA Polymerase
GC-Rich PCR Performance High
Fidelity (vs. Taq) 2 X
Product Type qPCR Master Mix
For Use With (Equipment) BioRad CFX96™, MJ Chromo4, MJ Opticon, Eppendorf MasterCycler RealPlex, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, Corbett RotorGene
Sample Type DNA, dsDNA
Reaction Speed Standard
Concentration 2X
For Use With (Application) Gene Expression
PCR Method qPCR
Product Line Luminaris
Detection Method SYBR
15

Invitrogen™ EXPRESS One-Step SYBR™ GreenER™ Kit, with premixed ROX

Polymerase Taq DNA Polymerase
Content And Storage EXPRESS One-Step SYBR™ GreenER™ SuperMix Kits are composed of 2 modules: EXPRESS SYBR™ GreenER™ RT module (containing SuperScript™ III Reverse Transcriptase and RNAseOUT™) and EXPRESS SYBR™ GreenER™ qPCR SuperMix with ROX premixed.

Store at -20°C upon receipt. Once thawed, the EXPRESS SYBR™ GreenER™ qPCR SuperMix can be stored for up to 1 month at +4·C. Product is guaranteed to be stable for 6 months when properly stored.
Format Tube
For Use With (Equipment) 7000 System, 7300 System, 7700 System, 7900HT Fast System, 7900HT System, Eppendorf™ MasterCycler™ RealPlex, StepOne™, Fast Mode, StepOne™, Standard Mode
Sample Type RNA
Reaction Speed Fast or Standard
For Use With (Application) Gene Expression
PCR Method 1-step RT-qPCR
Product Line SYBR™ GreenER™
Detection Method SYBR