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DMEM-F-12 Cell Culture Media

DMEM/F-12 cell culture media is a serum-free formulation that combines Dulbecco's modified Eagle medium (DMEM) and Ham's F-12 nutrient mixture in a 1:1 ratio. It is designed to provide a suitable environment for the growth and maintenance of various cell types in laboratory settings. This medium is enriched with essential nutrients, including insulin, transferrin, and growth factors, supporting the optimal proliferation and survival of cells1. DMEM/F-12 cell culture media has been widely used for culturing primary mammalian cells as well as various normal and transformed cell lines. Its versatility and ability to support a broad range of cell types make it a valuable tool in cellular research and biotechnological applications.
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1 – 15 of 21 results
1
Promotions Available

Gibco™ KnockOut™ DMEM/F-12
GREENER_CHOICE

KnockOut™ DMEM/F-12

2

Gibco™ DMEM/F-12, no phenol red
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DMEM/F-12, no phenol red

EDGE
3

Gibco™ DMEM/F-12
GREENER_CHOICE

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.

Shipping Condition Room Temperature
Cell Line MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts
Content And Storage Storage conditions: 2°C to 8°C (protect from light)
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Form Liquid
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Concentration 1 X
Sterility Sterile-filtered
Cell Type Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells
Sterilization Method Sterile-filtered
With Additives High Glucose,Glutamine,Phenol Red,Sodium Pyruvate
Product Type DMEM (Dulbecco's Modified Eagle Medium)/F-12
Classification Animal Origin-free
Shelf Life 12 Months From Date of Manufacture
Product Line Gibco
Serum Level Standard Serum Supplementation
4

Gibco™ Advanced DMEM/F-12
GREENER_CHOICE

Advanced DMEM/F-12 (Dulbecco's Modified Eagle Medium/Ham's F-12) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation.

Shipping Condition Room Temperature
Cell Line MRC-5, SP2, Vero, WI-38 and Jurkat
Content And Storage Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Form Liquid
Without Additives No Glutamine,No HEPES
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Concentration 1 X
Sterility Sterile-filtered
Sterilization Method Sterile-filtered
With Additives High Glucose,Phenol Red,Sodium Pyruvate,Non Essential Amino Acids (NEAA)
Product Type DMEM (Dulbecco's Modified Eagle Medium)/F-12
Classification Animal Origin
Shelf Life 12 Months From Date of Manufacture
Product Line Gibco
Serum Level Reduced Serum
5

Gibco™ DMEM/F-12, HEPES
GREENER_CHOICE

Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (DMEM / F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.

Sterilization Method Sterile-filtered
With Additives High Glucose,Glutamine,HEPES,Phenol Red,Sodium Pyruvate
Product Type DMEM (Dulbecco's Modified Eagle Medium)/F-12
Form Liquid
Classification Animal Origin-free
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Concentration 1 X
Sterility Sterile-filtered
6

Gibco™ DMEM/F-12, no phenol red
GREENER_CHOICE

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.

7

Gibco™ DMEM/F-12, HEPES
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DMEM/F-12, HEPES

Regulatory Status For in-vitro diagnostic use
Content And Storage 2 to 8°C
Description DMEM/F-12 Medium, 500mL, 1 X Concentration, Liquid Form, With L-glutamine, HEPES, Phenol Red, Sodium Pyruvate, 6.9 to 7.3 pH, 12 Months Shelf Life, 270 to 330mOsm/kg Osmolality, 2 to 8°C Storage Conditions, Used for Mammalian Cell Culture
With Additives Glutamine,HEPES,Phenol Red,Sodium Pyruvate
Product Type DMEM/F-12 Medium
Form Liquid
pH 6.9 to 7.3
Certifications/Compliance ISO 13485 standards, FDA
Shelf Life 12 Months
Concentration 1 X
For Use With (Application) Mammalian cell culture
8
Promotions Available

Gibco™ DMEM/F-12, GlutaMAX™ supplement
GREENER_CHOICE

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.

Sterilization Method Sterile-filtered
With Additives High Glucose,GlutaMAX,Phenol Red,Sodium Pyruvate
Product Type DMEM (Dulbecco's Modified Eagle Medium)/F-12
Form Liquid
Without Additives No HEPES
Classification Animal Origin-free
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Concentration 1 X
Sterility Sterile-filtered
9

Gibco™ DMEM/F-12, no glutamine
GREENER_CHOICE

Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (DMEM/F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.

Form Liquid
Without Additives No Glutamine,No HEPES
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Shelf Life 12 Months
10

Gibco™ DMEM/F-12
GREENER_CHOICE

DMEM/F-12

11

Gibco™ DMEM/F-12, HEPES, no phenol red
GREENER_CHOICE

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.

Cell Line MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts
Content And Storage Storage conditions: 2°C to 8°C (protect from light)
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Form Liquid
Without Additives No Phenol Red,No Sodium Pyruvate
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Concentration 1X
Sterility Sterile-filtered
Sterilization Method Sterile-filtered
With Additives High Glucose,Glutamine,HEPES
Product Type DMEM (Dulbecco's Modified Eagle Medium)/F-12
Classification Animal Origin-free
Shelf Life 12 Months From Date of Manufacture
Product Line Gibco
Serum Level Standard Serum Supplementation
12

Gibco™ BenchStable™ DMEM/F12
GREENER_CHOICE

Gibco DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.

Shipping Condition Room Temperature
Cell Line MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts
Content And Storage Store at room temperature. Protect from light.
Form Liquid
Without Additives No HEPES
Manufacturing Quality cGMP-compliant under the ISO 9001: 2015 standard
Green Features ACT Ecolabel (Silver)
Concentration 1 X
Sterility Sterile-filtered
With Additives High Glucose,GlutaMAX,Phenol Red,Sodium Pyruvate
Product Type DMEM (Dulbecco's Modified Eagle Medium)/F-12
Classification Animal Origin-free
Shelf Life 12 Months From Date of Manufacture
Product Line BenchStable
Serum Level Standard Serum Supplementation
13

Gibco™ DMEM/F-12, powder

DMEM/F-12, powder

Form Powder
Without Additives No HEPES,No Sodium Bicarbonate
pH 6.2 to 6.6
Certifications/Compliance ISO 13485 standards, FDA
Shelf Life 36 Months
For Use With (Application) Mammalian cell culture
14

Gibco™ SILAC Advanced DMEM/F-12 Flex Media, no glucose, no phenol red
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SILAC Advanced DMEM/F-12 Flex Media, no glucose, no phenol red