Test
Hydrolysis of substrate yields brightly fluorescent product
Analog of the natural substrate
Continuously assay acid phosphatases at low pH
Measure results in microplate assays or using flow cytometry
This Molecular Probes™ bisamide derivative of rhodamine 110 (R110) is a sensitive and selective substrate for assaying proteases in solution or inside living cells.
Resazurin, Sodium Salt
For the hydrolysis product, resorufin
Colorless and non-fluorescent
MUP is a fluorescent substrate for alkaline phosphatases
Novex™ AP Chemiluminescent Substrate (CDP-Star™)
Cleavage produces blue-fluorescence
Analog of natural substrate
The fluorogenic DQ™ collagen can be used to directly monitor collagenase activity.