Electrophoresis, Western Blotting and ELISA

Invitrogen™ NuPAGE™ Antioxidant

Proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis

Invitrogen™ NuPAGE™ MES SDS Running Buffer (20X)

Made with high-purity reagents and are strictly quality controlled

Invitrogen™ Mark12™ Unstained Standard

Allows the closest estimation of molecular weight of your sample protein over a wide molecular weight range

Thermo Scientific™ PageRuler™ Prestained Protein Ladder, 10 to 180 kDa

Mixture of 10 blue-, orange- and green-stained proteins (10 to 180kDa) used as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.

GE Healthcare pI Markers

Run these markers in parallel with the experimental samples on isoelectric focusing gels. GE Healthcare Life Sciences™ pI Markers are lyophilized mixtures of stable, salt-free, highly purified proteins.

Thermo Scientific™ SEA BLOCK Blocking Buffer

Block mammalian tissue samples for immunohistochemistry (IHC) and samples for other methods with steelhead salmon serum in PBS.

Thermo Scientific™ Blocker™ Casein

Block membranes, plates and other samples with these ready-to-use PBS or TBS solutions of purified casein for Western blot, ELISA and other detection methods.

Thermo Scientific™ PageRuler™ Plus Prestained Protein Ladder, 10 to 250 kDa

Mixture of 9 blue-, orange- and green-stained proteins (10 to 250kDa) used as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.

Invitrogen™ SimplyBlue™ SafeStain

Less hazardous and does not require methanol or acetic acid fixatives or destains

Invitrogen™ NuPAGE™ Sample Reducing Agent (10X)

Ready-to-use reagent to reduce protein samples for protein gel electrophoresis

Invitrogen™ eBioscience™ Permeabilization Buffer (10X)

Can be used to fix and permeabilize cells prior to performing intracellular staining of cytokines and other cytoplasmic and/or nuclear antigens.

Invitrogen™ eBioscience™ IC Fixation Buffer

Can be used to fix cells prior to permeabilization with the Permeabilization Buffer (cat. 00-8333) when performing intracellular staining of antigens located in the cytoplasm and secretory pathway, such as cytokines, chemokines and growth factors.

Invitrogen™ Novex™ Sharp Pre-stained Protein Standard

Designed for accurate, easy, and convenient molecular weight estimation of a wide range of molecular weight proteins during SDS-PAGE and Western blotting

Thermo Scientific™ Pierce™ Prestained Protein MW Marker

A mixture of six blue-stained natural proteins (20 to 120kDa) for use as size standards in protein electrophoresis (SDS-PAGE).

Thermo Scientific™ Pierce™ Silver Stain Kit

Stain proteins in 1D and 2D gels with this rapid, ultra-sensitive and versatile silver stain system that yields consistent and reliable results.

Thermo Scientific™ Sealing Tape for 96-Well Plates

Clear, self-sticking sealing tape for standard microplates.

Invitrogen™ TMB Stabilized Chromagen

For use with all sandwich ELISAs which utilize Horseradish Peroxidase (HRP) as detecting enzyme

Thermo Scientific™ Stop Solution for TMB Substrates

Stop TMB-based ELISA detection with this 0.16M sulfuric acid for use with the ELISA substrate 3,3',5,5' - tetramethylbenzidine (TMB).

Invitrogen™ SeeBlue™ Plus2 Pre-stained Protein Standard

Allows you to easily visualize protein molecular weight ranges during electrophoresis and quickly evaluate Western transfer efficiency

Invitrogen™ Bolt™ 4 to 12%, Bis-Tris, 1.0 mm Mini Protein Gel, 12-well

Separation Range: 3.5 to 260 kd; Separation Type: Denaturing; For Use With: Mini Gel Tank, XCell SureLock Mini

Invitrogen™ Bolt™ 12%, Bis-Tris, 1.0 mm, Mini Protein Gel, 10-well

Separation Range: 3.5 to 80 kd; Separation Type: Denaturing; For Use With: Mini Gel Tank, XCell SureLock Mini

Invitrogen™ NuPAGE™ MOPS SDS Running Buffer (20X)

Made with high-purity reagents and are strictly quality controlled

Invitrogen™ XCell SureLock™ Mini-Cell

Quickly and easily run up to two Novex 8 x 8cm minigels, leak-free without any clamps or grease

Invitrogen™ Bolt™ 12%, Bis-Tris, 1.0 mm, Mini Protein Gel, 15-well

Separation Range: 3.5 to 80 kd; Separation Type: Denaturing; For Use With: Mini Gel Tank, XCell SureLock Mini

Thermo Scientific™ SuperSignal™ West Pico PLUS Chemiluminescent Substrate

An enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram- to high femtogram-level protein detection by western blot analysis

BD TMB Substrate Reagent Set

For use in BD OptEIA™ ELISA sets. Substrate Reagent A, which is clear and colorless, contains hydrogen peroxide. Substrate Reagent B is colorless to a very light amber solution containing 3,3′, 5,5′ tetramethylbenzidine (TMB) in an organic solvent.

Thermo Scientific™ 1-Step™ TMB-Blotting Substrate Solution

Ready-to-use blue TMB peroxidase (HRP) substrate specially formulated for chromogenic detection in Western blot and IHC experiments.

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