Electrophoresis, Western Blotting and ELISA
Invitrogen™ NuPAGE™ Antioxidant
Proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis
Invitrogen™ NuPAGE™ MES SDS Running Buffer (20X)
Made with high-purity reagents and are strictly quality controlled
Invitrogen™ Mark12™ Unstained Standard
Allows the closest estimation of molecular weight of your sample protein over a wide molecular weight range
Invitrogen™ NuPAGE™ LDS Sample Buffer (4X)
Used to prepare protein samples for denaturing gel electrophoresis with NuPAGE™ Novex™ gels. Invitrogen™ Novex™ NuPAGE™ LDS Sample Buffer (4X) contains lithium dodecyl sulfate, pH 8.4, which allows for maximum activity of the reducing agent.
Thermo Scientific™ PageRuler™ Prestained Protein Ladder, 10 to 180 kDa
Mixture of 10 blue-, orange- and green-stained proteins (10 to 180kDa) used as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.
Cytiva pI Markers
Run these markers in parallel with the experimental samples on isoelectric focusing gels. Cytiva Life Sciences™ pI Markers are lyophilized mixtures of stable, salt-free, highly purified proteins.
Thermo Scientific™ Fish Serum Blocking Buffer
Block mammalian tissue samples for immunohistochemistry (IHC) and samples for other methods with steelhead salmon serum in PBS.
Thermo Scientific™ Blocker™ Casein in PBS
Block membranes, plates and other samples with these ready-to-use PBS solutions of purified casein for Western blot, ELISA, and other detection methods.
Thermo Scientific™ PageRuler™ Plus Prestained Protein Ladder, 10 to 250 kDa
Mixture of 9 blue-, orange- and green-stained proteins (10 to 250kDa) used as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.
Invitrogen™ SimplyBlue™ SafeStain
Less hazardous and does not require methanol or acetic acid fixatives or destains
Invitrogen™ NuPAGE™ Sample Reducing Agent (10X)
Ready-to-use reagent to reduce protein samples for protein gel electrophoresis
Invitrogen™ eBioscience™ Permeabilization Buffer (10X)
Can be used to fix and permeabilize cells prior to performing intracellular staining of cytokines and other cytoplasmic and/or nuclear antigens.
Invitrogen™ eBioscience™ IC Fixation Buffer
Can be used to fix cells prior to permeabilization with the Permeabilization Buffer (cat. 00-8333) when performing intracellular staining of antigens located in the cytoplasm and secretory pathway, such as cytokines, chemokines and growth factors.
Invitrogen™ Novex™ Sharp Pre-stained Protein Standard
Designed for accurate, easy, and convenient molecular weight estimation of a wide range of molecular weight proteins during SDS-PAGE and Western blotting
Thermo Scientific™ Pierce™ Prestained Protein MW Marker
A mixture of six blue-stained natural proteins (20 to 120kDa) for use as size standards in protein electrophoresis (SDS-PAGE).
Invitrogen™ ELISA TMB Stabilized Chromogen
For use with all sandwich ELISAs which utilize Horseradish Peroxidase (HRP) as detecting enzyme
Thermo Scientific™ Pierce™ Silver Stain Kit
Stain proteins in 1D and 2D gels with this rapid, ultra-sensitive and versatile silver stain system that yields consistent and reliable results.
Thermo Scientific™ Sealing Tape for 96-Well Plates
Clear, self-sticking sealing tape for standard microplates.
Thermo Scientific™ Stop Solution for TMB Substrates
Stop TMB-based ELISA detection with this 0.16M sulfuric acid for use with the ELISA substrate 3,3',5,5' - tetramethylbenzidine (TMB).
Invitrogen™ Bolt™ 4 to 12%, Bis-Tris, 1.0 mm, Mini Protein Gels
Invitrogen Bolt Bis-Tris Plus gels are precast polyacrylamide gels designed for optimal separation of a wide range of protein sizes under denaturing conditions.
Invitrogen™ SeeBlue™ Plus2 Pre-stained Protein Standard
Allows you to easily visualize protein molecular weight ranges during electrophoresis and quickly evaluate Western transfer efficiency
Invitrogen™ Bolt™ 12%, Bis-Tris, 1.0 mm, Mini Protein Gels
Invitrogen Bolt Bis-Tris Plus gels are precast polyacrylamide gels designed for optimal separation of a wide range of protein sizes under denaturing conditions. Bolt gels are ideal for western blot transfer and analysis, and any other techniques where protein integrity is crucial.
Invitrogen™ NuPAGE™ MOPS SDS Running Buffer (20X)
Made with high-purity reagents and are strictly quality controlled.
Invitrogen™ 20X Bolt™ MES SDS Running Buffer
Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats
Invitrogen™ XCell SureLock™ Mini-Cell
Quickly and easily run up to two Novex 8 x 8cm minigels, leak-free without any clamps or grease
Thermo Scientific™ SuperSignal™ West Pico PLUS Chemiluminescent Substrate
An enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram- to high femtogram-level protein detection by western blot analysis
BD TMB Substrate Reagent Set
For use in BD OptEIA™ ELISA sets. Substrate Reagent A, which is clear and colorless, contains hydrogen peroxide. Substrate Reagent B is colorless to a very light amber solution containing 3,3′, 5,5′ tetramethylbenzidine (TMB) in an organic solvent.
Thermo Scientific™ 1-Step™ TMB-Blotting Substrate Solution
Ready-to-use blue TMB peroxidase (HRP) substrate specially formulated for chromogenic detection in Western blot and IHC experiments.
Thermo Scientific™ 1-Step™ Ultra TMB-ELISA Substrate Solution
Horseradish peroxidase (HRP) substrates for ELISA that change from blue to yellow upon addition of a sulfuric or phosphoric acid stop solution.