Biological Buffers

Invitrogen™ NuPAGE™ Antioxidant

Invitrogen™ NuPAGE™ Antioxidant

Proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis

Invitrogen™ NuPAGE™ MES SDS Running Buffer (20X)

Invitrogen™ NuPAGE™ MES SDS Running Buffer (20X)

Made with high-purity reagents and are strictly quality controlled

Invitrogen™ NuPAGE™ LDS Sample Buffer (4X)

Invitrogen™ NuPAGE™ LDS Sample Buffer (4X)

Used to prepare protein samples for denaturing gel electrophoresis with NuPAGE™ Novex™ gels. Invitrogen™ Novex™ NuPAGE™ LDS Sample Buffer (4X) contains lithium dodecyl sulfate, pH 8.4, which allows for maximum activity of the reducing agent.

Thermo Scientific™ Fish Serum Blocking Buffer

Thermo Scientific™ Fish Serum Blocking Buffer

Block mammalian tissue samples for immunohistochemistry (IHC) and samples for other methods with steelhead salmon serum in PBS.

Thermo Scientific™ Blocker™ Casein in PBS

Thermo Scientific™ Blocker™ Casein in PBS

Block membranes, plates and other samples with these ready-to-use PBS solutions of purified casein for Western blot, ELISA, and other detection methods.

Invitrogen™ NuPAGE™ Sample Reducing Agent (10X)

Invitrogen™ NuPAGE™ Sample Reducing Agent (10X)

Ready-to-use reagent to reduce protein samples for protein gel electrophoresis

Invitrogen™ Nuclease-Free Water (not DEPC-Treated)

Invitrogen™ Nuclease-Free Water (not DEPC-Treated)

Nuclease-free water (not DEPC-treated) has been deionized, filtered into the final bottle, and autoclaved.

Invitrogen™ NuPAGE™ MOPS SDS Running Buffer (20X)

Invitrogen™ NuPAGE™ MOPS SDS Running Buffer (20X)

Made with high-purity reagents and are strictly quality controlled.

Invitrogen™ 20X Bolt™ MES SDS Running Buffer

Invitrogen™ 20X Bolt™ MES SDS Running Buffer

Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats

Thermo Scientific™ Guardian™ Peroxidase Conjugate Stabilizer/Diluent

Thermo Scientific™ Guardian™ Peroxidase Conjugate Stabilizer/Diluent

Preserve diluted (1-1,000ng/mL) HRP conjugates for more than six months at room temperature without significant loss of activity using Guardian Peroxidase Conjugate Stabilizer/Diluent.

Thermo Scientific™ SuperBlock™ T20 (PBS) Blocking Buffer

Thermo Scientific™ SuperBlock™ T20 (PBS) Blocking Buffer

Block membranes, plates and tissue samples with these optimized solutions containing a protein that provides fast and effective blocking for Western blot, ELISA and IHC.

Gibco™ HEPES (1M)

Gibco™ HEPES (1M)

A zwitterionic organic chemical buffering agent commonly used in cell culture media

Thermo Scientific™ SuperBlock™ T20 (TBS) Blocking Buffer

Thermo Scientific™ SuperBlock™ T20 (TBS) Blocking Buffer

Block membranes, plates and tissue samples with these optimized solutions containing a protein that provides fast and effective blocking for Western blot, ELISA and IHC.

Invitrogen™ EDTA (0.5 M), pH 8.0, RNase-free

Invitrogen™ EDTA (0.5 M), pH 8.0, RNase-free

Ready-to-use, certified RNase-free

Invitrogen™ Glycogen (5 mg/ml)

Invitrogen™ Glycogen (5 mg/ml)

Enhances precipitation of nucleic acids

Invitrogen™ TE, pH 8.0, RNase-free

Invitrogen™ TE, pH 8.0, RNase-free

Ready-to-use, certified RNase-free

Invitrogen™ ATP Solution (10 mM)

Invitrogen™ ATP Solution (10 mM)

Also for use in ligation and phosphorylation reactions

Invitrogen™ NativePAGE™ Running Buffer Kit

Invitrogen™ NativePAGE™ Running Buffer Kit

Contains the NativePAGE Running Buffer (20X)

Thermo Scientific™ MgCl<sub>2</sub> (magnesium chloride) (25 mM)

Thermo Scientific™ MgCl2 (magnesium chloride) (25 mM)

Optimize your PCR reaction conditions with this Magnesium Chloride (MgCl2) solution.

Invitrogen™ NativePAGE™ Sample Buffer (4X)

Invitrogen™ NativePAGE™ Sample Buffer (4X)

A solution used to solubilize native protein samples prior to use with the NativePAGE Gel System

Invitrogen™ RT-PCR Grade Water

Invitrogen™ RT-PCR Grade Water

Ready to use — no mixing or autoclaving

Invitrogen™ Novex™ Tris-Glycine SDS Running Buffer (10X)

Invitrogen™ Novex™ Tris-Glycine SDS Running Buffer (10X)

Formulated for separation of proteins, in their denatured state, on Novex Tris-Glycine gels

Invitrogen™ NaCl (5 M), RNase-free

Invitrogen™ NaCl (5 M), RNase-free

Ready-to-use, certified RNase-free

Invitrogen™ Yeast tRNA (10 mg/mL)

Invitrogen™ Yeast tRNA (10 mg/mL)

Co-precipitant to aid in nucleic acid recovery

Thermo Scientific™ Phire Reaction Buffer

Thermo Scientific™ Phire Reaction Buffer

Thermo Scientific™ Phire Reaction Buffer is a reaction buffer for Phire DNA Polymerases.

Invitrogen™ Tris (1 M), pH 7.0, RNase-free

Invitrogen™ Tris (1 M), pH 7.0, RNase-free

Ready-to-use, certified RNase-free

Invitrogen™ Bolt™ Transfer Buffer (20X)

Invitrogen™ Bolt™ Transfer Buffer (20X)

Optimized for the transfer of proteins from Bolt Bis-Tris Plus gels to membranes for western blotting

Invitrogen™ Acid-Phenol:Chloroform, pH 4.5 (with IAA, 125:24:1)

Invitrogen™ Acid-Phenol:Chloroform, pH 4.5 (with IAA, 125:24:1)

Aids in the removal of DNA (partitions into the organic phase), helps to stabilize the interface, and prevents foaming when mixing in RNA extraction procedures.

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