Enzymen en remmers

Thermo Scientific™ DNase I, RNase-vrij

An endonuclease that nonspecifically cleaves single- and double-stranded DNA to release di-, tri-, and oligonucleotides with 5'-phosphate and 3'-OH groups. DNase I, RNase-free, 50 u/µl, 1000 u

Thermo Scientific™ ATP

Optimize many molecular biology applications using this ATP, a highly stable nucleotide with 99% purity. ATP, 99% purity by HPLC, 100 mM, 0.25 ml

Thermo Scientific™ Exonuclease I (Exo I)

Degrade single-stranded DNA in a 3'→5' direction with Exonuclease I (ExoI), leaving 5'-terminal dinucleotides intact. Exonuclease I (Exo I), 20 u/µl, 20000 u, with 5x1ml of 10X Reaction Buffer

Thermo Scientific™ 50X TAE-buffer (Tris-acetaat-EDTA)

Optimize electrophoresis of both genomic and large supercoiled DNA in agarose and polyacrylamide gels with Thermo Scientific™ 50X TAE Buffer (Tris-acetate-EDTA). 50X TAE Buffer (Tris-acetate-EDTA), 1 L

Thermo Scientific™ FastDigest™ BamHI

Cut at G^GATCC sites with Thermo Scientific™ FastDigest™ BamHI restriction enzyme, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer. X5 FASTDIGEST BAMHI 500UL

Thermo Scientific™ RNase A, DNase en protease-vrij

Specifically degrade single-stranded RNA at C and U residues with the endoribonuclease RNase A. RNase A, DNase and protease-free, 10 mg/ml, 10 mg

Lipase AY30, ACROS Organics

CAS: 9001-62-1 25GR Lipase AY30

Thermo Scientific™ FastAP thermosensitieve alkalische fosfatase

Catalyze the removal of 5'- and 3'-phosphate groups from DNA, RNA, nucleotides, and proteins using this thermosensitive alkaline phosphatase . FastAP Thermosensitive Alkaline Phosphatase, 1 u/µl, 5x1000 u

Thermo Scientific™ Runderserumalbumine (BSA)

Stabilize enzymes, increase PCR yields, and prevent adhesion of enzymes with Thermo Scientific™ Bovine Serum Albumin. Bovine Serum Albumin (BSA), 20 mg/ml, 5 mg (0.25 ml)

Invitrogen™ RNase Cocktail Enzyme Mix

Endonuclease-free, exonuclease-free, protease-free RNAse Cocktail 5 X 1 ml Each

Thermo Scientific™ NotI

The NotI restriction enzyme recognizes GC^GGCCGC sites and cuts best at 37°C in O buffer (Isoschizomers: CciNI). NotI, 10 u/µl, 500 u, cuts GC¬GGCCGC at 37°C

Thermo Scientific™ FastDigest™ NcoI

The FastDigest NcoI restriction enzyme recognizes C^CATGG sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: Bsp19I). FastDigest NcoI, 100 ul, cuts C¬CATGG, 1µl cleaves 1µg DNA in 5 min at 37°C

Invitrogen™ TURBO DNA-free Kit

Ideal tool for clearing residual DNA Turbo DNAfree 50 rxns Each

Invitrogen™ UltraPure™ Glycogen

Used as carrier for nucleic acid precipitation in ethanol 100 uL, GLYCOGEN

Fisher BioReagents™ Proteinase K (Tritirachium album/Molecular Biology)

50MG Proteinase K, from Tritirachium album, DNaseand RNase free, for Molecular Biology

Thermo Scientific™ Eco47III (AfeI)

The Eco47III (AfeI) restriction enzyme recognizes AGC^GCT sites and cuts best at 37°C in O buffer (Isoschizomers: AfeI, Aor51HI). Eco47III (AfeI), 10 u/µl, 1000 u, cuts AGC¬GCT at37°C

Thermo Scientific™ DNase I, RNase-vrij

Endonuclease that nonspecifically cleaves single- and double-stranded DNA to release di-, tri-, and oligonucleotides with 5'-phosphate and 3'-OH groups. DNase I, RNase-free, 1 u/µl, 1000 u

Thermo Scientific™ FastDigest BsmBI (Esp3I)

Cut at CGTCTC(1/5)^ sites with Thermo Scientific™ FastDigest™ BsmBI (Esp3I), which peforms best at 37°C in 5-15 minutes using universal FastDigest Buffer. FastDigest BsmBI (Esp3I), 20 ul, cuts CGTCTC(1/5)¬

Thermo Scientific™ BshNI (BanI)

Cut at G^GYRCC sites with BshNI (BanI) restriction enzyme, which performs best at 37°C in O buffer (Isoschizomers: AccB1I, BanI, BspT107I). BshNI (BanI), 10 u/µl, 2000 u, cuts G¬GYRCC at 37°C

Thermo Scientific™ FastDigest™ NotI

The FastDigest NotI restriction enzyme recognizes GC^GGCCGC sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: CciNI). FastDigest NotI 250 rxn, 1µl cleaves 1µg DNA in 5min at 37°C

Thermo Scientific™ FastDigest AvrII (XmaJI)

Cut at C^CTAGG sites with Thermo Scientific™ FastDigest™ AvrII (XmaJI), which performs best using universal FastDigest Buffer (Isoschizomers: AspA2I, BlnI). FastDigest AvrII (XmaJI), 20 ul, cuts C¬CTAGG, 1 µl cleaves 1µg DNA 5 min at 37°C

Thermo Scientific™ NdeI

The NdeI restriction enzyme recognizes CA^TATG sites and cuts best at 37°C in O buffer (Isoschizomers: FauNDI). NDEI 10U/UL 4000U

Thermo Scientific™ T4-polynucleotidekinase (T4 PNK)

T4 Polynucleotide Kinase (T4 PNK) catalyzes the transfer of the gamma-phosphate from ATP to the 5'-OH group of oligonucleotides, ss and dsDNA and RNA. T4 Polynucleotide Kinase (T4 PNK), 10 u/µl, 2500 u

Thermo Scientific™ FastDigest BbsI (BpiI)

Cut at GAAGAC(2/6)^ sites with Thermo Scientific™ FastDigest™ BbsI (BpiI), which performs best using universal FastDigest Buffer (Isoschizomers: BpuAI, BstV2I). FastDigest BbsI (BpiI), 20 ul, cuts GAAGAC(2/6)¬

Thermo Scientific™ PsyI (Tth111I)

Cut at GACN^NNGTC sites with PsyI (Tth111I) restriction enzyme, which functions best at 37°C in B buffer (Isoschizomers: AspI, PflFI, Tth111I). PsyI (Tth111I), 10 u/µl, 1000 u, cuts GACN¬NNGTC at 37°C

Thermo Scientific™ 10x Buffer Tango

Ensure the optimum reaction conditions for restriction enzymes with Thermo Scientific™ 10X Buffer Tango, premixed with BSA for enhanced stability. X5 10X Buffer Tango Unit Size 5x1.0 mL

Thermo Scientific™ AluI

Cut at AG^CT sites with AluI restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: AluBI). AluI, 10 u/µl, 600 u, cuts AG¬CT at 37°C, supplied with 1 ml of 10X Buffer Tango

Thermo Scientific™ PstI

The PstI restriction enzyme recognizes CTGCA^G sites and cuts best at 37°C in O buffer (Isoschizomers: BspMAI). PstI, 10 u/µl, 5x3000 u, cuts CTGCA¬G at 37°C

Thermo Scientific™ PvuI

The PvuI restriction enzyme recognizes CGAT^CG sites and cuts best at 37°C in R buffer (Isoschizomers: BpvUI, MvrI, Ple19I). PvuI, 10 u/µl, 1500 u, cuts CGAT¬CG at 37°C

Thermo Scientific™ 10X reactiebuffer met MgCl2 voor DNase I

Optimize reaction condions for RNase-free DNase I with this 10X reaction Tris-HCl (pH 7.5) buffer containing MgCl2. 10x Reaction buffer with MgCl2 for DNase I

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