Enzymen en remmers

Thermo Scientific™ ATP

Optimize many molecular biology applications using this ATP, a highly stable nucleotide with 99% purity. ATP, 99% purity by HPLC, 100 mM, 0.25 ml

Thermo Scientific™ Exonuclease I (Exo I)

Degrade single-stranded DNA in a 3'→5' direction with Exonuclease I (ExoI), leaving 5'-terminal dinucleotides intact. Exonuclease I (Exo I), 20 u/µl, 20000 u, with 5x1ml of 10X Reaction Buffer

Lipase AY30, ACROS Organics

CAS: 9001-62-1 25GR Lipase AY30

Thermo Scientific™ RNase A, DNase en protease-vrij

Specifically degrade single-stranded RNA at C and U residues with the endoribonuclease RNase A. RNase A, DNase and protease-free, 10 mg/ml, 10 mg

Invitrogen™ DNase I, Amplification Grade

Purified and tested for nondetectable levels of RNase contamination DNase I, Amplification Grade, 100 units

Thermo Scientific™ 50X TAE-buffer (Tris-acetaat-EDTA)

Optimize electrophoresis of both genomic and large supercoiled DNA in agarose and polyacrylamide gels with Thermo Scientific™ 50X TAE Buffer (Tris-acetate-EDTA). 50X TAE Buffer (Tris-acetate-EDTA), 1 L

Thermo Scientific™ DNase I, RNase-vrij

An endonuclease that nonspecifically cleaves single- and double-stranded DNA to release di-, tri-, and oligonucleotides with 5'-phosphate and 3'-OH groups. DNase I, RNase-free, 50 u/µl, 1000 u

Invitrogen™ Clonase™ Gateway™ LR Clonase II Enzyme Mix

Enzymes and buffer in single mix 100 RXN, LR CLONASE II ENZYME MIX

Thermo Scientific™ T4-polynucleotidekinase (T4 PNK)

T4 Polynucleotide Kinase (T4 PNK) catalyzes the transfer of the gamma-phosphate from ATP to the 5'-OH group of oligonucleotides, ss and dsDNA and RNA. T4 Polynucleotide Kinase (T4 PNK), 10 u/µl, 2500 u

Thermo Scientific™ NotI

The NotI restriction enzyme recognizes GC^GGCCGC sites and cuts best at 37°C in O buffer (Isoschizomers: CciNI). NotI, 50 u/µl, HC, 1500 u, cuts GC¬GGCCGC at 37°C

Thermo Scientific™ FastDigest BbsI (BpiI)

Cut at GAAGAC(2/6)^ sites with Thermo Scientific™ FastDigest™ BbsI (BpiI), which performs best using universal FastDigest Buffer (Isoschizomers: BpuAI, BstV2I). FastDigest BbsI (BpiI), 20 ul, cuts GAAGAC(2/6)¬

Thermo Scientific™ FastDigest™ XbaI

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer. FastDigest XbaI, 750ul, cuts T¬CTAGA, 1µl cleaves1µg DNA in 5 min at 37°C

Thermo Scientific™ FastAP thermosensitieve alkalische fosfatase

Catalyze the removal of 5'- and 3'-phosphate groups from DNA, RNA, nucleotides, and proteins using this thermosensitive alkaline phosphatase . FastAP Thermosensitive Alkaline Phosphatase, 1 u/µl, 5x1000 u

Thermo Scientific™ AluI

Cut at AG^CT sites with AluI restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: AluBI). AluI, 10 u/µl, 600 u, cuts AG¬CT at 37°C, supplied with 1 ml of 10X Buffer Tango

Thermo Scientific™ T4 DNA-ligase

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. T4 DNA Ligase, 5 u/µl, 5x1000 u

Thermo Scientific™ Runderserumalbumine (BSA)

Stabilize enzymes, increase PCR yields, and prevent adhesion of enzymes with Thermo Scientific™ Bovine Serum Albumin. Bovine Serum Albumin (BSA), 20 mg/ml, 5 mg (0.25 ml)

Invitrogen™ DNA-free™ DNA Removal Kit

Safely eliminate DNA contamination from RNA samples DNAfree 50 rxns Each

Thermo Scientific™ FastDigest BsmBI (Esp3I)

Cut at CGTCTC(1/5)^ sites with Thermo Scientific™ FastDigest™ BsmBI (Esp3I), which peforms best at 37°C in 5-15 minutes using universal FastDigest Buffer. FastDigest BsmBI (Esp3I), 20 ul, cuts CGTCTC(1/5)¬

Thermo Scientific™ FastDigest™ EcoRI

The FastDigest EcoRI restriction enzyme recognizes G^AATTC sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer. X5 FASTDIGEST ECORI 500UL

Thermo Scientific™ Eco47III (AfeI)

The Eco47III (AfeI) restriction enzyme recognizes AGC^GCT sites and cuts best at 37°C in O buffer (Isoschizomers: AfeI, Aor51HI). Eco47III (AfeI), 10 u/µl, 1000 u, cuts AGC¬GCT at37°C

Invitrogen™ Clonase™ Gateway™ BP Clonase II Enzyme Mix

Catalyzes in vitro recombination of PCR products 100 RXN, BP CLONASE II ENZYME MIX

Thermo Scientific™ FastDigest KpnI

Perform single-, double- or multiple DNA digestion within 5-15 minutes withthis advanced line of restriction enzymes, 100% active in one universal buffer. FastDigest KpnI, 300 ul, cuts GGTAC¬C, 1µl cleaves 1µg DNA in 5 min at 37°C

Invitrogen™ Clonase™ Gateway™ LR Clonase II Plus enzyme

Enzyme mix that offers high recombination efficiency 100 RXNS LR CLONASE II PLUS ENZYME

Thermo Scientific™ FastDigest DdeI (HpyF3I)

Cut at C^TNAG sites with Thermo Scientific™ FastDigest™ DdeI (HpyF3I), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: BstDEI). FastDigest DdeI (HpyF3I), 50 ul, cuts C¬TNAG, 1 µl cleaves 1µg DNA 5 min at 37°C

Thermo Scientific™ FastDigest™ BamHI

Cut at G^GATCC sites with Thermo Scientific™ FastDigest™ BamHI restriction enzyme, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer. X5 FASTDIGEST BAMHI 500UL

Thermo Scientific™ NdeI

The NdeI restriction enzyme recognizes CA^TATG sites and cuts best at 37°C in O buffer (Isoschizomers: FauNDI). NDEI 10U/UL 4000U

MP Biomedicals™ Diaphorase from Clostridium kluyveri

Used to assess the protein-protein interactions in assembly of lipoic acid on the 2-oxoacid dehydrogenases of aerobic metabolism 1.8KU DIAPHORASE

Thermo Scientific™ DNase I, RNase-vrij

Endonuclease that nonspecifically cleaves single- and double-stranded DNA to release di-, tri-, and oligonucleotides with 5'-phosphate and 3'-OH groups. DNase I, RNase-free, 1 u/µl, 1000 u

Thermo Scientific™ FastDigest™ HindIII

The FastDigest HindIII restriction enzyme recognizes A^AGCTT sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer. X5 FASTDIGEST HINDIII 500UL

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