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R&D Systems™ Recombinant Human Active MEK1 Kinase Protein, CF
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:
20 μg
Description
Measured by its ability to hydrolyze the 5’-phosphate groups from the substrate adenosine-5’-triphosphate (ATP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit (Catalog # DY996 ). The specific activity is >7 pmol/min/μg, as measured under the described conditions.
Specifications
Specifications
| Accession Number | Q02750.2 |
| For Use With (Application) | Enzyme Activity |
| Formulation | Supplied as a 0.2 μm filtered solution in Tris, NaCl, DTT and Glycerol. |
| Gene ID (Entrez) | 5604 |
| Molecular Weight (g/mol) | M.W.-observed: 62-68 kDa, under reducing conditions, M.W.-theroretical: 69 kDa |
| Quantity | 20 μg |
| Source | Spodoptera frugiperda, Sf 21 (baculovirus)-derived human MEK1 protein Met1-Val393 with an N-terminal GST tag |
| Storage Requirements | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening. |
| Endotoxin Concentration | <0.10 EU per 1 μg of the protein by the LAL method. |
| Gene Alias | dual specificity mitogen-activated protein kinase kinase 1, ERK activator kinase 1, MAP kinase kinase 1, MAP2K1, MAPK/ERK kinase 1, MAPKK1, MEK1MAPKK 1, mitogen-activated protein kinase kinase 1, MKK1, MKK1MEK 1, PRKMK1EC 2.7.12.2, protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1) |
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