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TdB Labs AB 400 kDa FITC-polysucrose Green Fluorophore
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Quantity:
100 mg
1 g
Unit Size:
100mg
1g
Description
- Product Overview: FITC-polysucrose is a high molecular weight, water-soluble fluorescent tracer based on polysucrose labeled with fluorescein isothiocyanate (FITC). Offering bright green fluorescence and excellent biocompatibility, FITC-polysucrose is ideal for studies involving permeability, molecular diffusion, vascular barrier function, and biological transport models. Its stability and non-toxicity make it suitable for both live-cell experiments and tissue models.
- Features:
- Green Fluorescence: Excitation maximum at 495 nm and emission maximum at 519 nm, compatible with standard FITC filter sets.
- High Molecular Weight Options: Available in different sizes (e.g., 20 kDa, 40 kDa, 70 kDa, 100 kDa, 400 kDa) to match diffusion and permeability requirements.
- Water-Soluble and Biocompatible: Fully soluble in aqueous solutions, suitable for biological systems without inducing cytotoxicity.
- Bright and Stable Labeling: FITC conjugation provides durable fluorescence for both real-time tracking and fixed endpoint studies.
- Minimal Non-Specific Binding: Maintains clear background and reliable signal detection in complex biological environments.
- Benefits:
- Reliable Molecular Diffusion Tracking: Models macromolecule behavior across biological barriers and in engineered systems.
- Ideal for Permeability Assays: Commonly used to assess tight junction integrity, vascular leakage, and epithelial barrier function.
- Supports Live-Cell and Fixed-Cell Imaging: Suitable for both dynamic real-time experiments and post-fixation endpoint analysis.
- Flexible Use in Diverse Systems: Applied in microfluidic platforms, organ-on-chip models, perfused tissues, and 3D constructs.
- Bright Fluorescence for Easy Quantification: Ensures sensitive and reproducible detection across imaging and plate reader platforms.
- Applications:
- Permeability and Barrier Integrity Studies: Used in epithelial, endothelial, and blood-brain barrier models to assess transport properties.
- Tracer Studies in Microfluidics and Bioreactors: Visualizes molecular transport in flow-based systems.
- Live and Fixed Imaging Applications: Tracks distribution of macromolecules in live-cell or fixed-tissue experiments.
- Diffusion and Transport Modeling: Supports research into size-dependent molecular movement in biological and synthetic systems.
- Technical Specifications:
- Fluorescence: Excitation maximum at 495 nm, emission maximum at 519 nm.
- Molecular Weights: Available in multiple sizes, including 20 kDa, 40 kDa, 70 kDa, 100 kDa, and 400 kDa.
- Appearance: Light-sensitive, greenish dry powder, readily soluble in water.
- Labeling: Optimized degree of labeling to ensure bright fluorescence while maintaining polysucrose behavior.
- Storage and Stability:
- Store dry and protected from light at 2°C to 8°C for optimal stability and fluorescence retention.
- Stable under recommended conditions for extended periods without significant loss of performance.
- Packaging and Availability:
- Supplied as dry powder to maximize stability and handling ease.
- Available in multiple research-grade packaging sizes to support both small-scale and larger experimental needs.
Specifications
Specifications
| Detection Method | Fluorescence |
| Format | Vial |
| Product Type | Reagent |
| Quantity | 100 mg |
| Form | Crystalline Powder |
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