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Novus Biologicals™ Recombinant Human IGF-I/IGF-1 LR3 Protein

Product Code. 18640648 Shop All Bio Techne Products
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Product Code. 18640648

Brand: Novus Biologicals™ NBP2613145mg

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Description

Recombinant Protein

Assay 1: Fully biologically active when compared to standard. Measured in a serum-free cell proliferation assay using human MCF-7 cells. The ED50 for this effect is 0.3-1.5 ng/mL, corresponding to a specific activity of > 6.7 x 10^5 U/mg. Assay 2: Fully biologically active when compared to standard. The ED50 as determined by the stimulation of protein synthesis using rat L6 myoblasts is less than 10 ng/mL, corresponding to a specific activity of > 1.0 x 10^5 U/mg.
TRUSTED_SUSTAINABILITY

Specifications

For Use With (Application) SDS-PAGE, Bioactivity
Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.2.
Gene ID (Entrez) 3479
Molecular Weight (g/mol) MolecularWeight-theroretical: 9.1 kDa
Quantity 5 mg
Storage Requirements Store at -20°C to -70°C as supplied. After reconstitution, store at 2 to 8 C for 1 month and (at -20°C to -70°C for long term storage. Avoid repeated freeze/thaw cycles.)
Endotoxin Concentration Less than 0.01 EU/μg of IGF-I/IGF-1 AS determined by LAL method.
Gene Alias IBP1, IGF1A, IGFI, IGF-I, IGF-IA, IGF-IB, insulin-like growth factor 1, insulin-like growth factor 1 (somatomedin C), insulin-like growth factor I, insulin-like growth factor IA, insulin-like growth factor IB, Mechano growth factor, MGF, somatomedin-C
Gene Symbol IGF1
Reconstitution Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL Apportion stock solutions into working aliquots and store (at <-20°C.)
Research Category Biologically Active Proteins, Cell Cycle and Replication, Signal Transduction
Purity or Quality Grade >98%, by SDS-PAGE
Protein IGF-I/IGF-1
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