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Invitrogen™ A-Raf Monoclonal Antibody (M289)
Mouse Monoclonal Antibody
Brand: Invitrogen™ MA547730
This item is not returnable.
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Description
This antibody detects a 68 kDa* protein corresponding to the molecular mass of A-Raf on SDS-PAGE immunoblots of human SKN-SH and Jurkat cells.
Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. A-Raf, a second member of the Raf gene family of serine/ threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of A-Raf is found at highest levels in urogenital tissues and kidney and at lowest level in brain tissue.
Specifications
| A-Raf | |
| Monoclonal | |
| 0.125 mg/mL | |
| PBS with 1mg/mL BSA, 50% glycerol and 0.05% sodium azide | |
| P04627, P10398, P14056 | |
| ARAF | |
| Clone (M289) was generated from a sequence corresponding to amino acids in the N-terminal region of human A-Raf. This A-Raf sequence has high homology to rat and mouse A-Raf. | |
| 100 μL | |
| Primary | |
| Human, Mouse, Rat | |
| Antibody | |
| IgG1 |
| Western Blot | |
| M289 | |
| Unconjugated | |
| ARAF | |
| 1200013E08Rik; Araf; A-RAF; A-Raf proto-oncogene serine/threonine-protein kinase; A-Raf proto-oncogene, serine/threonine kinase; Araf1; A-raf-1; AW495444; Oncogene ARAF1; PKS; PKS2; Proto-oncogene A-Raf; proto-oncogene A-Raf-1; Proto-oncogene Pks; raf a; Raf related protein; RAFA1; raf-related oncogene; Ras-binding protein; Ras-binding protein DA-Raf; RP1-230G1.1; serine/threonine-protein kinase A-Raf; v-raf murine sarcoma 3611 viral oncogene homolog; v-raf murine sarcoma 3611 viral oncogene homolog 1; v-raf murine sarcoma 3611 viral oncogene-like protein; v-raf oncogene homolog 1 (murine sarcoma 3611 virus) | |
| Mouse | |
| Protein G | |
| RUO | |
| 11836, 369, 64363 | |
| -20°C, Avoid Freeze/Thaw Cycles | |
| Liquid |
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