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Invitrogen™ AccuCheck Counting Beads

Product Code. 10110533
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10 mL
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10110533 10 mL Each
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Product Code. 10110533 Supplier Invitrogen™ Supplier No. PCB100

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Description

AccuCheck Counting Beads

AccuCheck Counting Beads are an efficient single-platform method for absolute cell counts that combines the advantages of direct flow cytometric immunophenotyping with the use of two different fluorescent beads (A and B beads). These two fluorospheres are used as a double internal standard for blood volume calculation. A known volume of AccuCheck Counting Beads is added to the same known volume of stained blood in a lyse-no-wash technique. The beads are counted along with cells. Since the concentration of beads is known, the number of cells per microliter (the absolute count) is obtained by relating the number of cells counted to the total number of fluorescent bead events. The cell number is then multiplied by the number of total fluorospheres per unit of volume. As the AccuCheck Counting Beads system contains two different fluorospheres in a known proportion, we can first assure the accuracy of the assay by verifying the proportion of both types of beads.

TRUSTED_SUSTAINABILITY

Specifications

Diameter (Metric) 6.36 μm, 6.4 μm
Content And Storage Contains 1 bottle of AccuCheck counting beads (10 mL). Store in refrigerator (2–8°C).
Emission 488 and 633/635
Format Solution
For Use With (Equipment) Flow Cytometer
No. of Tests 100 tests
Quantity 10 mL
Detection Method Fluorescence
Excitation Wavelength Range Bead A: 488/575-585 nm, Bead B: 635/660-680 nm
Shipping Condition Wet Ice
Product Type Cell Counting Bead
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Frequently Asked Questions (FAQs)

I am using counting beads to count cells, but I cannot find the beads on my scatter plots. What do I do?

The first thing to do is check your threshold and see if it is set on forward scatter. If so, the beads are probably being excluded by the threshold. Reducing the threshold setting should reveal your beads.

I want to count my cells using flow cytometry. How do I do this?

Cell counting using flow cytometry can be accomplished by adding an internal microsphere counting standard to the flow cytometric sample. The number of reference beads that are collected reflects a known volume. This allows you to calculate cell concentration.

For Research Use Only. Not for use in diagnostic procedures.

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