Invitrogen™ ALKBH5 Polyclonal Antibody

Description

Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL. Positive Control - WB: human 293T whole cell, human Jurkat whole cell, human Hela whole cell, human HepG2 whole cell, human U87 whole cell. ICC/IF: A549 cell. Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

ALKBH5 (RNA demehtylase ALKBH5) is a dioxygenase that demethylates RNA by oxidative demethylation. Specifically, it demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes. Demethylation of m6A mRNA affects mRNA processing and export. ALKBH5 can also demethylate N(6)-methyladenosine in single-stranded DNA (in vitro). It requires molecular oxygen, alpha-ketoglutarate and iron.