Invitrogen™ Allopregnanolone ELISA Kit

ELISA

The Allopregnanolone Competitive ELISA quantitates allopregnanolone in serum, plasma, urine, saliva, dried fecal extracts or cell culture medium.∣Principle of the method: The Progesterone Metabolites Competitive ELISA research-use-only kit is designed to quantitatively measure allopregnanolone independent of species. A allopregnanolone standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with anti-rabbit IgG antibodies. Anti-rabbit allopregnanolone antibodies and allopregnanolone-peroxidase conjugate is added to the wells. The allopregnanolone-peroxidase conjugate and any allopregnanolone in the sample will compete to bind to the anti-rabbit allopregnanolone antibodies. After incubation, the plate is washed and substrate is added. The substrate reacts with the bound allopregnanolone-peroxidase conjugate. After a 30 min incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader at 450 nm. The intensity of the generated color corresponds inversely to the amount of allopregnanolone present in the sample.∣Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.