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Invitrogen™ PureLink™ Pro 96 total RNA Purification Kit
Isolate high yields of total RNA with low genomic DNA contamination from bacteria, yeast, plant, or mammalian samples.
Brand: Invitrogen™ 12173011A
Additional Details : Weight : 0.56000kg
Description
Isolate high yields of total RNA with low genomic DNA contamination from bacteria, yeast, plant, or mammalian samples.
The one-hour procedure requires no handling or disposal of organic waste. RNA binds to a silica membrane and impurities are removed by washing through vacuum filtration or centrifugation. High-purity total RNA is eluted into RNase-free water; ideal for use in a variety of applications, including RT-PCR, qPCR, northern blotting, and nuclease protection assays.
Improved plate design
The PureLink™ Pro 96 total RNA Purification Kit provides optimized semi-skirted 96-well plates for improved performance and results. In addition, the plate nozzles with annular collar prevent cross-contamination of samples and improve drying of the silica membrane to prevent ethanol carryover. In addition, sample processing can be performed manually using a benchtop vacuum manifold, by centrifugation (at 2250 x g), or with an automated liquid handling platform. The semi-skirted plate design is compatible with most vacuum manifolds on robotic workstations.
Specifications
• 150 mL PureLink Pro 96 Lysis Buffer; store at room temperature • 250 mL PureLink Pro 96 Wash Buffer I; room temperature • 250 mL PureLink Pro 96 Wash Buffer II; room temperature • 75 mL PureLink Pro 96 RNase-free Water; room temperature • 4 PureLink 96-well Total RNA Filter Plates; room temperature • 4 PureLink Pro 96 Elution Plates; room temperature |
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25 min. (After sample homogenization) | |
Bacteria, Cells, Plant, Tissue, Yeast | |
High-throughput Compatible | |
RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling | |
Mini | |
Bacteria: ≤109 cells Cells: varies Plant: ≤150 mg Tissue: ≤100 mg Yeast: ≤1.8 mL |
Silica Plate | |
45–170 μL | |
Total RNA | |
Biomek FX, Robotic Liquid Handling Systems, Eppendorf epMotion TECAN Freedom Evo, TECAN Genesis | |
4 Plates | |
Room Temperature | |
Up to 33 μg |
Safety and Handling
- PureLink Pro 96 total RNA Purification Kit
Signal Word
- Danger
Hazard Category
- Acute toxicity Category 4
- LONG-TERM AQUATIC HAZARD Chronic 3
- Serious eye damage/eye irritation Category 1
- Serious eye damage/eye irritation Category 2
- Skin corrosion/irritation Category 1 B
- Skin corrosion/irritation Category 2
Hazard Statement
- H302-Harmful if swallowed.
- H314-Causes severe skin burns and eye damage.
- H315-Causes skin irritation.
- H318-Causes serious eye damage.
- H319-Causes serious eye irritation.
- H412-Harmful to aquatic life with long lasting effects.
Precautionary Statement
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P264-Wash hands thoroughly after handling.
- P270-Do not eat, drink or smoke when using this product.
- P273-Avoid release to the environment.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
- P301+P330+P331-IF SWALLOWED: rinse mouth. Do NOT induce vomiting.
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P310-Immediately call a POISON CENTER/doctor/.
- P330-Rinse mouth.
- P332+P313-If skin irritation occurs: Get medical advice/attention.
- P337+P313-If eye irritation persists: Get medical advice/attention.
- P362+P364-Take off contaminated clothing and wash it before reuse.
- P363-Wash contaminated clothing before reuse.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : Protein denaturant,Thiocyanic acid
For Research Use Only. Not for use in diagnostic procedures.