Invitrogen™ ULTRAhyb™-Oligo

• Contains a proprietary blend of hybridization accelerators and blocking agents to enhance the levels of hybridization
• Supplied in one bottle containing 125mL
• Use less probe per blot
• Decrease hybridization time to 2 hours
• Using standard hybridization buffers, only 1 to 5% of target molecules on a blot hybridizes to probe, making blots a relatively insensitive method for nucleic acid analysis (Vernier et al. (1996) Anal Biochem 235: 11-19)
• With ULTRAhyb™ Ultrasensitive Hybridization Buffer, the hybridization reaction approaches completion, so that as few as 10,000 molecules can be detected
• It works well with oligonucleotides having T_ms from 78° to 90°C and 47 to 62% GC contents; However, the optimal hybridization temperature for oligonucleotide probes with different GC content and T_m values should be determined empirically

DNA and RNA Purification and Analysis, Northern Blotting, Nucleic Acid Gel Electrophoresis and Blotting