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Description
The HIT3a monoclonal antibody specifically binds to the human CD3 ε-chain, a 20 kDa subunit of the CD3/T cell antigen receptor complex found on 70-80% of normal human peripheral blood lymphocytes and 60-85% of thymocytes. Studies from the HLDA Workshop show that this antibody can be mitogenic for T lymphocytes. The CD3 complex plays a role in signal transduction during antigen recognition by the T cell receptor for antigen. HIT3a antibody does not stain intracellular CD3 unlike the other CD3-specific clone, UCHT1.
This antibody is conjugated to BD Horizon BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set. BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605-nm. Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers. BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser.
Specifications
| CD3 | |
| Monoclonal | |
| Brilliant Violet 605 | |
| Aqueous buffered solution containing BSA and ≤0.09% sodium azide. | |
| Affinity Purified | |
| RUO | |
| Human | |
| IgG2a κ |
| Flow Cytometry | |
| HIT3a | |
| CD3E; T3E; TCRE; cd 3; cd-3; cd3; CD3-epsilon; 916 | |
| Mouse | |
| 100 Tests | |
| Primary | |
| Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. |
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