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CD3e Hamster anti-Mouse, V450, Clone: 500A2, BD
Hamster Monoclonal Antibody
Brand: BD Biosciences 560801
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Description
This antibody has been reported to react with the 25-kDa ε chain of the T-cell receptor-associated CD3 complex expressed on thymocytes, mature T lymphocytes, and NK-T cells on many mouse stains. Plate-bound and soluble hamster anti-mouse CD3e (clone 500A2) antibody has been reported to activate unprimed T cells in vitro. Activation of a mouse T-cell clone by soluble hamster anti-mouse CD3e (clone 500A2) can be blocked by Fab fragments of anti-CD4 antibody GK1.5, suggesting that the hamster anti-mouse CD3e (clone 500A2) antibody may bind an epitope of CD3e close to a site at which CD4 associates with the T-cell receptor. This hamster mAb to a mouse leukocyte antigen has been reported not to cross-react with rat leukocytes.
The antibody is conjugated to BD Horizon™ V450, which has been developed for use in multicolor flow cytometry experiments and is available exclusively from BD Biosciences. It is excited by the 450 nm. Conjugates with BD Horizon™ V450 can be used in place of Pacific Blue™ conjugates.
Specifications
| CD3e | |
| Monoclonal | |
| 0.2mg/mL | |
| CD3ε chain | |
| Hamster | |
| Affinity Purified | |
| RUO | |
| Mouse | |
| IgG2 κ |
| Flow Cytometry | |
| 500A2 | |
| Violet Fluor 450 (V450) | |
| Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide. | |
| Mouse T-cell receptor | |
| 0.1 mg | |
| Primary | |
| Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. |
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