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CD45 Mouse, Brilliant Violet 786, Clone: D058-1283, BD
Mouse Monoclonal Antibody
Brand: BD Biosciences 563861
Description
D058-1283 is a CD45 monoclonal antibody specific for non-human primate leucocytes, developed using Rhesus peripheral whole blood as immunogen. It does not cross react with human leucocytes. This antibody reacts with Baboon, Rhesus and Cynomolgus Macaque leucocytes in a similar pattern as seen with CD45 binding to the Leukocyte Common Antigen on human cells. Immunophenotypic analysis shows that D058-1283 binds to lymphocytes, monocytes and granulocytes of non-human primate blood samples. This antibody is able to block the binding of monoclonal antibody TÜ116; a reported anti-human CD45 antibody that cross-reacts with nonhuman primate leucocytes. In Western blot analysis, the D058-1283 antibody identifies a band of approximately 180-200 kDa molecular weight .
The antibody was conjugated to BD Horizon™ BV786 which is part of the BD Horizon™ Brilliant Violet™ family of dyes. This dye is a tandem fluorochrome of BD Horizon™ BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 786-nm. BD Horizon™ BV786 can be excited by the violet laser and detected in a filter used to detect Cy7™-like dyes (eg, 780/60-nm filter).
Specifications
| CD45 | |
| Monoclonal | |
| Brilliant Violet 786 | |
| Aqueous buffered solution containing BSA and ≤0.09% sodium azide. | |
| Rhesus peripheral whole blood | |
| 50 Tests | |
| Primary | |
| Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. |
| Flow Cytometry | |
| D058-1283 | |
| Pan Leukocyte, NHP-specific | |
| Mouse | |
| Affinity Purified | |
| RUO | |
| Baboon, Cynomolgus, Rhesus Monkey | |
| IgG1 κ |
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