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IgE Rat anti-Mouse, Brilliant Violet 650, Clone: R35-72, BD

Product Code. 15813449
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50 μg
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Product Code. 15813449 Supplier BD Biosciences Supplier No. 564208

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Description

Rat Monoclonal Antibody

The rat anti-mouse IgE antibody (clone R35-72) reacts specifically with mouse IgE of the Igh-C [a] and Igh-C [b] haplotypes. It has been reported not to react with other Ig isotypes. Detection with the rat anti-mouse IgE antibody (clone R35-72) of surface immunoglobulin on IgE-secreting hybridoma cells has also been reported.

The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant Violet™ family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm. BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors. However, the spillover can be corrected through compensation as with any other dye combination.

TRUSTED_SUSTAINABILITY

Specifications

Antigen IgE
Applications Flow Cytometry
Classification Monoclonal
Clone R35-72
Concentration 0.2mg/mL
Conjugate Brilliant Violet 650
Description Igh-7; immunoglobulin heavy chain 7 (heavy chain of IgE)
Host Species Rat
Immunogen Mouse IgE (pooled)
Purification Method Affinity Purified
Quantity 50 μg
Regulatory Status RUO
Primary or Secondary Primary
Target Species Mouse
Content And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Isotype IgG1 κ
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