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Description
Clone MQ1-17H12 reacts with the human form of interleukin-2 (IL-2) detectable in the cytoplasm of a major subset of activated peripheral blood T lymphocytes, following treatment with monensin or brefeldin A. Clone MQ1-17H12 also cross-reacts with a cytoplasmic component of peripheral blood CD3+ lymphocytes of baboon, and both rhesus and cynomolgus macaque monkeys following six-hour treatment with phorbol myristic acetate (PMA) and Ca++ ionophore (A23187) in the presence of monensin. While the frequency of CD3+ non-human primate cells reacting with MQ1-17H12 following PMA + Ca++ ionophore activation is similar to that observed with normal human donor CD3+ lymphocytes, the fluorescence intensity is characteristically weaker. This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
Intracellular Staining
Specifications
| IL-2 | |
| Monoclonal | |
| FITC | |
| Rat | |
| Affinity Purified | |
| RUO | |
| Primary | |
| Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. |
| Flow Cytometry | |
| MQ1-17H12 | |
| Aqueous buffered solution containing BSA and ≤0.09% sodium azide. | |
| Recombinant Human IL-2 | |
| 50 Tests | |
| Immunology | |
| Baboon, Cynomolgus, Rhesus Monkey | |
| IgG2a κ |
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