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  6. p27[Kip1] Mouse, Unlabeled, Clone: 57, BD

p27[Kip1] Mouse, Unlabeled, Clone: 57, BD

Product Code. 15815359
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150 μg
50 μg
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15815359 50 μg 1 set
15825359 150 μg 1 set
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Product Code. 15815359 Supplier BD Biosciences Supplier No. 610241

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Description

Mouse Monoclonal Antibody

Kip1 is a cyclin-dependent kinase (cdk) inhibitor that was identified as a result of its role in TGFβ-induced G1 phase arrest and cell-cell contact. In vitro , p27 [Kip1] binds tightly to Cyclin D-Cdk4, Cyclin E-Cdk2, and Cyclin A-Cdk2 complexes and inhibits their activity. In normal cells, Kip1 is sequestered and its activity gradually decreases as cells reach S phase. The addition of TGF-β in early G1 blocks this decline in activity, by preventing the sequestration of Kip1. TGF-β also reduces the levels of Cdk4. Kip1 preferentially binds Cyclin D-Cdk4, but the lower levels of Cdk4 in TGF-β treated cells allow Kip1 to be available for binding to Cyclin E-Cdk2 and Cyclin A-Cdk2. Kip1 is structurally related to Cip1/WAF1, having a similar 60 amino acid sequence in the N-terminal region. A 52 amino acid Kip1 peptide (residues 28-79) from this region is sufficient to inhibit cdk activity in vitro.

Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting

TRUSTED_SUSTAINABILITY

Specifications

Antigen p27[Kip1]
Applications Western Blot
Classification Monoclonal
Clone 57
Concentration 250μg/mL
Conjugate Unconjugated
Formulation Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
Host Species Mouse
Immunogen Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
Purification Method Affinity Purified
Quantity 50 μg
Regulatory Status RUO
Research Discipline Cell Biology
Primary or Secondary Primary
Target Species Canine, Chicken, Frog, Human, Mouse, Rat
Content And Storage Store undiluted at -20°C.
Isotype IgG1
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For Research Use Only.

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