missing translation for 'onlineSavingsMsg'
Learn More

Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Buffer I

AmpliTaq Gold™ DNA Polymerase is a chemically modified form of AmpliTaq™ DNA Polymerase requiring thermal activation.

Brand:  Applied Biosystems™ N8080240

267.90 EUR valid until 2024-12-31
Use promo code "21967" to get your promotional price.



Additional Details : Weight : 0.01000kg

 View more versions of this product

Product Code. 10026054

  • € 295.00 / Each
In Stock
to see stock.

Description

Description

AmpliTaq Gold™ DNA Polymerase is a chemically modified form of AmpliTaq™ DNA Polymerase requiring thermal activation.

Features of this enzyme:

  • Automated chemical hot-start enzyme for increased specificity, sensitivity, and yield
  • Time-released thermal activation improves sensitivity in low copy number amplifications
  • Successful multiplex PCR saves time and reagents
  • Includes GeneAmp™ 10X PCR Buffer I containing 15 mM MgCl2

Hot-start activation

The modified enzyme is provided in an inactive state. Upon thermal activation, the modifier is permanently released, regenerating active enzyme. The resulting hot-start PCR amplification provides increased sensitivity, specificity, and yield over conventional PCR techniques.

Higher specificity, higher yield

AmpliTaq Gold™ DNA Polymerase can be activated partially or completely in a pre-PCR heat step, or can be allowed to activate slowly in a time-released manner during the denaturation steps of thermal cycling. With or without a limited up-front heat activation step, active enzyme is released slowly during thermal cycling to match template concentration and increase specificity. The yield of specific product increases because reactants are not wasted in the formation of unintended products. Because AmpliTaq Gold™ DNA Polymerase is a chemical hot-start enzyme, there is no worry of biological contamination.

AmpliTaq Gold™ 360 DNA Polymerase for superior performance

Also available is AmpliTaq Gold™ 360 DNA Polymerase for even better PCR performance. Compared to AmpliTaq Gold™ DNA Polymerase, AmpliTaq Gold™ 360 DNA Polymerase is purified by an additional proprietary separation process to eliminate contaminating bacterial DNA sequences from the enzyme preparation. When used with the enhanced 360 Gold Buffer, this ultra-pure enzyme, in addition to its hot-start capabilities, reduces false positive results, amplifies low-level target sequences, and promotes the amplification of a variety of templates. Also included with this product is a 360 GC Enhancer to work through challenging GC-rich templates.

Specifications

Specifications

• AmpliTaq Gold DNA Polymerase (5 U/μL), 1 x 50 μL
• 10X PCR Buffer I (with MgCl2), 1 x 1.5 mL

Store at -15°C to -30°C.
Low
Standard
DNA Polymerase With Buffer I
Dry Ice
5 U/μL
Built-In Hot Start
3'-A
5 kb or less
Tube
AmpliTaq Gold DNA Polymerase
5' - 3'
250 Units
Hot-start PCR
1X
200 Reactions
Separate Components
DNA
Product Suggestions

Product Suggestions

Videos
SDS
Documents

Documents

Certificates
Special Offers

Special Offers

For Research Use Only. Not for use in diagnostic procedures.