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Armenian Hamster IgG Isotype Control (eBio299Arm), eFluor™ 450, eBioscience™, Invitrogen™
Armenian Hamster Isotype Control
€ 92.50 - € 210.00
Specifications
Antigen | Armenian Hamster IgG |
---|---|
Clone | eBio299Arm |
Concentration | 0.2 mg/mL |
Applications | Control, Flow Cytometry |
Conjugate | eFluor 450 |
Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
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Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
15331780
|
Affymetrix eBioscience
48-4888-80 |
25 μg |
€ 92.50
25µg |
Estimated Shipment: 09-05-2024
Log in to see stock. |
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15341780
|
Affymetrix eBioscience
48-4888-82 |
100 μg |
€ 210.00
100µg |
In Stock
Log in to see stock. |
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Description
Description: The eBio299Arm monoclonal antibody is useful as an isotype control immunoglobulin. Applications Reported: This eBio299Arm antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBio299Arm antibody has been tested by flow cytometric analysis of mouse splenocytes. Use isotype control at the same concentration as experimental antibody. eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser. Filtration: 0.2 μm post-manufacturing filtered.
The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.Specifications
Armenian Hamster IgG | |
0.2 mg/mL | |
eFluor 450 | |
Armenian Hamster | |
Not Applicable | |
IgG; Immunoglobulin G; ImmunoglobulinG | |
Isotype Control (Primary) | |
4° C, store in dark, DO NOT FREEZE! |
eBio299Arm | |
Control, Flow Cytometry | |
Liquid | |
RUO | |
PBS with 0.09% sodium azide; pH 7.2 | |
IgG | |
Affinity chromatography |
For Research Use Only.