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BD Human TNF ELISA Kit II
For quantitative determination of human TNF in serum , plasma, and cell culture supernatant
Brand: BD 550610
Additional Details : Weight : 1.00000kg
Description
The OptEIA test is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay).
- Utilizes monoclonal antibody specific for TNF coated on a 96-well plate
- Developed for accuracy with serum and plasma specimens
ELISA
Includes:
- Antibody Coated Wells: 2 plates of 96 breakable wells (12 strips × 8 wells) coated with anti-human TNF monoclonal antibody
- Detection Antibody: 30mL of biotinylated anti-human TNF monoclonal antibody containing FBS and ProClin™-150 as preservative
Standards: 4 vials lyophilized recombinant human TNF - Enzyme Concentrate: 150μL of 250× concentrated Streptavidinhorseradish peroxidase conjugate with BSA and ProClin-300 as preservative
- Standard/Sample Diluent: 30mL of animal serum with 0.09% sodium azide as preservative
- ELISA Diluent: 12mL of a buffered protein base with 0.09% sodium azide as preservative
- Wash Concentrate (20×): 100mL of 20× concentrated detergent solution
- TMB One-Step Substrate Reagent: 30 ml of 3,3',5,5'-tetramethylbenzidine (TMB) in buffered solution
- Stop Solution: 13mL of 1 M phosphoric acid
- Plate Sealers: 4 sheets with adhesive backing
Specifications
| TNF | |
| Human TNF Antibody coated plate, detection Antibody Biotin Anti-Human TNF, Human TNF standard, Enzyme concentrate Streptavidin -HRP, wash concentrate (20X), TMB one-step Substrate reagent, stop solution, standard/Sample Diluent, ELISA Diluent | |
| Serum, Plasma, Cell Culture Supernatant | |
| Quantitative determination of Human TNF, ELISA |
| Human | |
| 2 Plates | |
| 2°C to 8°C | |
| Human TNF ELISA kit II |
Research Use Only