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BD Human TNF ELISA Kit II

Product Code. 12774138
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2 Plates
Unit Size:
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12774138 2 Plates Each
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Product Code. 12774138 Supplier BD Supplier No. 550610

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Description

For quantitative determination of human TNF in serum , plasma, and cell culture supernatant

The OptEIA test is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay).

  • Utilizes monoclonal antibody specific for TNF coated on a 96-well plate
  • Developed for accuracy with serum and plasma specimens

ELISA

Includes:

  • Antibody Coated Wells: 2 plates of 96 breakable wells (12 strips × 8 wells) coated with anti-human TNF monoclonal antibody
  • Detection Antibody: 30mL of biotinylated anti-human TNF monoclonal antibody containing FBS and ProClin™-150 as preservative
    Standards: 4 vials lyophilized recombinant human TNF
  • Enzyme Concentrate: 150μL of 250× concentrated Streptavidinhorseradish peroxidase conjugate with BSA and ProClin-300 as preservative
  • Standard/Sample Diluent: 30mL of animal serum with 0.09% sodium azide as preservative
  • ELISA Diluent: 12mL of a buffered protein base with 0.09% sodium azide as preservative
  • Wash Concentrate (20×): 100mL of 20× concentrated detergent solution
  • TMB One-Step Substrate Reagent: 30 ml of 3,3',5,5'-tetramethylbenzidine (TMB) in buffered solution
  • Stop Solution: 13mL of 1 M phosphoric acid
  • Plate Sealers: 4 sheets with adhesive backing
TRUSTED_SUSTAINABILITY

Specifications

Antigen TNF
Cross Reactivity Human
Includes Human TNF Antibody coated plate, detection Antibody Biotin Anti-Human TNF, Human TNF standard, Enzyme concentrate Streptavidin -HRP, wash concentrate (20X), TMB one-step Substrate reagent, stop solution, standard/Sample Diluent, ELISA Diluent
Quantity 2 Plates
Sample Type Serum, Plasma, Cell Culture Supernatant
Content And Storage 2°C to 8°C
For Use With (Application) Quantitative determination of Human TNF, ELISA
Product Type Human TNF ELISA kit II

Safety and Handling

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Product Identifier
  • BD OptEIA Human TNF ELISA Kit II
 Signal Word
  • Danger
 Hazard Category
  • Serious eye damage/eye irritation Category 1
  • Skin corrosion/irritation Category 1 B
  • Skin sensitiser Category 1
 Hazard Statement
  • H314-Causes severe skin burns and eye damage.
  • H317-May cause an allergic skin reaction.
  • H318-Causes serious eye damage.
 Precautionary Statement
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
  • P264-Wash thoroughly after handling.
  • P272-Contaminated work clothing should not be allowed out of the workplace.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P330+P331-IF SWALLOWED: rinse mouth. Do NOT induce vomiting.
  • P302+P352-IF ON SKIN: Wash with plenty of water/.
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P310-Immediately call a POISON CENTER/doctor/.
  • P321-Specific treatment (see on this label).
  • P333+P313-If skin irritation or rash occurs: Get medical advice/attention.
  • P362+P364-Take off contaminated clothing and wash it before reuse.
  • P405-Store locked up.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Supplemental information
  • MIXTURE LIST-Contain: 5-chloro-2-methyl-4-isothiazolin3-one, orthophosphoric acid

Research Use Only

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