PeproTech C19orf10 Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Description

AA Sequence of recombinant protein: MVSEPTTVPF DVRPGGVVHS FSQDVGPGNK FTCTFTYASQ GGTNEQWQMS LGTSEDSQHF TCTIWRPQGK SYLYFTQFKA ELRGAEIEYA MAYSKAAFER ESDVPLKSEE FEVTKTAVSH RPGAFKAELS KLVIVAKAAR SEL. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine SF-20. Anti-Murine SF-20-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect mSF-20 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine SF-20 (500-P259) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant mSF-20. Western Blot: To detect mSF-20 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant mSF-20 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P259BT-1MG will be provided as 2 x 500 μg

The interleukins are a broad family of well characterized cytokines, primarily of hematopoietic cell origin. Cytokines are small, soluble proteins with pleiotropic effects on a variety of cell types. Cytokines have a regulatory function over the immune system and mediate aspects of inflammatory response. IL-25 (also called SF20/IL-25) is a secreted bone marrow stroma-derived growth factor cytokine that is derived from Th2 T-cells. IL-25 belongs to the IL-17 cytokine family and is capable of amplifying allergic type inflammatory responses by its actions on other cell types. IL-25 binds to mouse thymic shared antigen-1 and supports lymphoid cell proliferation. Infusion of mice with IL-25 induces IL-4, IL-5, and IL-13 gene expression. IL-25 induced mice also develop epithelial cell hyperplasia, increased mucus secretion, and airway hyperreactivity, suggesting that IL-25 may be an important mediator of allergic disease via production of IL-4, IL-5, IL-13 and eotaxin.