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  6. Invitrogen™ CCL2 (MCP-1) Monoclonal Antibody (2H5), Biotin, eBioscience™

Invitrogen™ CCL2 (MCP-1) Monoclonal Antibody (2H5), Biotin, eBioscience™

Product Code. p-7168037
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Quantity:
500 μg
Unit Size:
500µg
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Product Code. Quantity unitSize
15340030 500 μg 500µg
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Product Code. 15340030 Supplier Invitrogen™ Supplier No. 13709685

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Description

Armenian Hamster Monoclonal Antibody

Description: The 2H5 monoclonal antibody reacts with mouse, rat, and human monocyte chemoattractant protein-1 (MCP-1), also known as CCL2 and MCAF. Applications Reported: The 2H5 antibody has been reported for use in ELISA, intracellular staining for flow cytometric analysis, and cytokine neutralization. (Please use Functional Grade purified 2H5, Product # 16-7096, in functional assays). Applications Tested: Mouse MCP-1 ELISA: The biotinylated 2H5 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of mouse MCP-1 in combination with the affinity purified 4E2/MCP (Product # 14-7091-81) antibody for capture and recombinant mouse MCP-1 as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2.0 μg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/mL - 15 pg/mL should be included in each ELISA plate. Human MCP-1 ELISA: The biotinylated 2H5 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of human MCP-1 in combination with the affinity purified 5D3-F7 (Product # 14-7099-81) antibody for capture and recombinant human MCP-1 as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2.0 μg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/mL - 15 pg/mL should be included in each ELISA plate. The Functional Grade purifie...

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.
TRUSTED_SUSTAINABILITY

Specifications

Antigen CCL2 (MCP-1)
Applications ELISA, Flow Cytometry
Classification Monoclonal
Clone 2H5
Concentration 0.5 mg/mL
Conjugate Biotin
Formulation PBS with 0.09% sodium azide; pH 7.2
Gene Ccl2
Gene Accession No. P10148, P13500, P14844
Gene Alias Acidic seminal fluid protein; AI323594; C-C motif chemokine 2; C-C motif chemokine ligand 2; Ccl2; chemokine (C-C motif) ligand 2; GDCF-2; HC11; H-MCP-1; HSMCR30; immediate-early serum-responsive JE protein; immediate-early serum-responsive protein JE; Je; MCAF; Mcp1; MCP-1; MCP1A; MCP-1A; MGC9434; Monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; monocyte chemotactic and activating factor; Monocyte chemotactic protein 1; monocyte chemotactic protein 1A; Monocyte secretory protein JE; monocyte-chemoattractant protein-1 precursor; platelet-derived growth factor-inducible protein JE; RP23-350G1.3; SCYA2; Sigje; small inducible cytokine A2; small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je); small inducible cytokine subfamily A (Cys-Cys), member 2; small inducible gene JE; small-inducible cytokine A2; SMC-CF
Gene Symbols Ccl2
Host Species Armenian Hamster
Purification Method Affinity chromatography
Quantity 500 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 20296, 24770, 6347
Target Species Human, Mouse, Rat
Content And Storage 4°C, store in dark, DO NOT FREEZE!
Product Type Antibody
Form Liquid
Isotype IgG
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