Invitrogen™ CD11b Monoclonal Antibody (ICRF44), Brilliant Ultra Violet™ 661, eBioscience™, Invitrogen™

Description

The ICRF44 monoclonal antibody reacts with human CD11b, a 165 kDa adhesion molecule. CD11b associated with integrin beta2 (CD18) is expressed on the surface of monocytes, granulocytes, activated lymphocytes and a subset of NK cells. CD11b is a receptor for intercellular adhesion molecule family members CD54, CD102 and CD50 as well as for iC3b. These adhesions are crucial in cell-cell and cell-matrix interactions. Applications Reported: This ICRF44 antibody has been reported for use in flow cytometric analysis. Applications Tested: This ICRF44 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 μL (0.5 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Brilliant Ultraviolet™ 661 (BUV661) is a tandem dye that emits at 660 nm and is intended for use on cytometers equipped with an ultraviolet (355 nm) laser. Fixation: Samples can be stored in IC Fixation Buffer (00-8222-49) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Excitation: 350 nm; Emission: 660 nm; Laser: Ultraviolet Laser.

CD11b Monoclonal antibody specifically detects CD11b in Baboon, Chimpanzee, Cynomolgus Monkey, Human, Rhesus Monkey samples. It is validated for Flow Cytometry.