CD142 Mouse anti-Human, Brilliant Violet 510, Clone: HTF-1, BD Optibuild™

Description

The HTF-1 monoclonal antibody specifically binds to CD142. CD142 is a 45-47 kDa, single chain, type I transmembrane glycoprotein also known as Tissue Factor (TF). CD142 has been referred in the literature as coagulation Factor III or thromboplastin and it is expressed on activated endothelial cells and lipopolysaccharide (LPS)-stimulated monocytes/macrophages. TF associates with factor VIIa to form a complex and acts as an enzyme that initiates the blood coagulation cascade. It is known as the major initiator of clotting in normal hemostasis. CD142 can be induced by various inflammatory mediators in monocytes and vascular endothelial cells. This antibody is useful in inflammation, thrombosis and hemostasis research.

The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 405nm and Em Max at 510nm, BD Horizon BV510 can be excited by the violet laser and detected in the BD Horizon V500 (525/50nm) filter set. BD Horizon BV510 conjugates are useful for the detection of dim markers off the violet laser. BV510 is a polymer-based dye that is brighter than BD Horizon V500. Due to similar excitation and emission properties, BD Horizon BV510 and BD Horizon V500 cannot be used simultaneously.