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  6. Invitrogen™ CD170 (Siglec F) Monoclonal Antibody (1RNM44N), NovaFluor™ Blue 800, eBioscience™

Invitrogen™ CD170 (Siglec F) Monoclonal Antibody (1RNM44N), NovaFluor™ Blue 800, eBioscience™

Product Code. p-200108975
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Quantity:
100 μg
25 μg
Unit Size:
25µg
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Product Code. Quantity unitSize
30283524 25 μg 25µg
30283522 100 μg -
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Product Code. 30283524 Supplier Invitrogen™ Supplier No. M042T02B12A

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Description

Rat Monoclonal Antibody

Description: This 1RNM44N monoclonal antibody recognizes mouse CD170, also known as Siglec F. Mouse Siglec F is a functional ortholog of human Siglec 8, however, unlike human Siglec 8, mouse Siglec F is not expressed on mast cells. This 1RNM44N antibody will recognize a formaldehyde-fixed epitope. This product contains 1 vial of NovaFluor conjugate and 1 vial of CellBlox Plus Blocking Buffer. Applications Tested: This 1RNM44N antibody has been tested by flow cytometric analysis of mouse thioglycolate-elicited peritoneal exudate cells. This can be used at less than or equal to 0.06 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest Master mixes. Whole Blood compatibility: When utilizing whole blood (as opposed to density-gradient-purified PBMC), we recommend lysing red blood cells in bulk prior to staining with NovaFluor conjugates.

SIGLEC5 is a putative adhesion molecule that mediates sialic-acid dependent binding to cells. This protein binds equally to alpha2,3-linked and alpha2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. This molecule is expressed by monocytic/myeloid lineage cells. SIGLEC5 is found at high levels in peripheral blood leukocytes, spleen, bone marrow and at lower levels in lymph node, lung, appendix, placenta, pancreas and thymus. It is expressed by monocytes and neutrophils but absent from leukemic cell lines representing early stages of myelomonocytic differentiation.
TRUSTED_SUSTAINABILITY

Specifications

Antigen CD170 (Siglec F)
Applications Flow Cytometry
Classification Monoclonal
Clone 1RNM44N
Concentration 0.1 mg/mL
Conjugate NovaFluor Blue 800
Formulation PBS with BSA and 0.09% sodium azide; pH 7.2
Gene Siglecf
Gene Accession No. Q920G3
Gene Alias CD170; CD33 antigen-like 2; CD33L2; mSiglec-F; OB-binding protein 2; OBBP2; OB-BP2; Obesity-binding protein 2; sialic acid binding Ig like lectin 5; sialic acid binding Ig-like lectin 5; sialic acid binding Ig-like lectin F; sialic acid-binding Ig-like lectin 5; Sialic acid-binding Ig-like lectin F; sialic acid-binding immunoglobulin-like lectin 5; sialic acid-binding lectin Siglec-F; Siglec; SIGLEC5; siglec-5; Siglecf; Siglec-F
Gene Symbols Siglecf
Host Species Rat
Purification Method Affinity chromatography
Quantity 25 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 233186
Target Species Mouse
Content And Storage 4°C, store in dark, DO NOT FREEZE!
Product Type Antibody
Form Liquid
Isotype IgG2a κ
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