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CD19, PE-eFluor 610, clone: eBio1D3 (1D3), eBioscience™

Rat Monoclonal Antibody

Merk:  Affymetrix eBioscience 61-0193-80

Overige gegevens : Gewicht : 0.09500kg

 Bekijk meerdere types van dit product via de

Produktcode. 15540417

  • € 96.25 / 25 µg


Geschatte verzenddatum
bij leverancier 17-08-2018
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Beschrijving

Beschrijving

Description: The eBio1D3 (1D3) monoclonal antibody reacts with mouse CD19, a 95kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express CD19. Together CD21, CD81, MHC class II, and CD19 form a multimolecular complex that associates with the BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells.Applications Reported: This eBio1D3 (1D3) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBio1D3 (1D3) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.PE-eFluor® 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red®). Please make sure that your instrument is capable of detecting this fluorochome.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specificatie

Specificatie

CD19
Monoclonal
0.2mg/mL
PBS with 0.09% sodium azide; pH 7.2
P25918
AW495831, B-lymphocyte antigen CD19, differentiation antigen CD19
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
eBio1D3 (1D3)
PE-eFluor 610
Cd19
Liquid
Cd19
IgG2a, kappa, kappa
25μg
Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling and experimental procedures.
Primary
12478
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For Research Use Only.