CD196 (CCR6), eFluor 660, clone: sirx6, eBioscience™
Rat Monoclonal Antibody
Merk: Affymetrix eBioscience 50-7196-82
Overige gegevens : Gewicht : 0.25000kg
BeschrijvingDescription: The sirx6 monoclonal antibody reacts with mouse CD196 (CCR6). CD196 is a seven transmembrane G protein-coupled receptor expressed on T, B, dendritic, NK and Langerhans cells. This CC chemokine receptor uniquely binds MIP3a/CCL20, a chemoattractant for dendritic cells, effector/memory T cells, and B cells. CD196 is also involved in host defense and inflammation at epithelial sites. Furthermore, this receptor has been implicated in Th17 differentiation and CD4+FoxP3+ regulatory T cell development.Due to expression characteristics of some chemokine receptors (shedding, surface cleavage or intracellular cycling), we recommend a fixation step after surface staining. Refer to Applications Tested for more information.Applications Reported: This sirx6 antibody has been reported for use in flow cytometric analysis.Applications Tested: This sirx6 antibody has been tested by flow cytometric analysis of mouse splenocytes. Cells are surface stained, then washed with Flow Cytometry Staining Buffer, immediately fixed with IC Fixation Buffer for 20-30 minutes, and then washed with Flow Cytometry Staining Buffer prior to analysis. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor™ 660 is a replacement for Alexa Fluor™ 647. eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|BN-1, CKR-L3, CKR6, CKRL3, CMKBR6, DCR2, DRY-6, GPR-CY4, GPR29, GPRCY4, STRL22|
|IgG2a, kappa, kappa|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.