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CD196 (CCR6), eFluor 660, clone: sirx6, eBioscience™

Rat Monoclonal Antibody

Merk:  Affymetrix eBioscience 50-7196-82

Overige gegevens : Gewicht : 0.25000kg

 Bekijk meerdere types van dit product via de

Produktcode. 15331950

  • € 259.00 / 100 µg


Geschatte verzenddatum
bij leverancier 24-08-2018
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Beschrijving

Beschrijving

Description: The sirx6 monoclonal antibody reacts with mouse CD196 (CCR6). CD196 is a seven transmembrane G protein-coupled receptor expressed on T, B, dendritic, NK and Langerhans cells. This CC chemokine receptor uniquely binds MIP3a/CCL20, a chemoattractant for dendritic cells, effector/memory T cells, and B cells. CD196 is also involved in host defense and inflammation at epithelial sites. Furthermore, this receptor has been implicated in Th17 differentiation and CD4+FoxP3+ regulatory T cell development.Due to expression characteristics of some chemokine receptors (shedding, surface cleavage or intracellular cycling), we recommend a fixation step after surface staining. Refer to Applications Tested for more information.Applications Reported: This sirx6 antibody has been reported for use in flow cytometric analysis.Applications Tested: This sirx6 antibody has been tested by flow cytometric analysis of mouse splenocytes. Cells are surface stained, then washed with Flow Cytometry Staining Buffer, immediately fixed with IC Fixation Buffer for 20-30 minutes, and then washed with Flow Cytometry Staining Buffer prior to analysis. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
Specificatie

Specificatie

CD196 (CCR6)
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
O54689
BN-1, CKR-L3, CKR6, CKRL3, CMKBR6, DCR2, DRY-6, GPR-CY4, GPR29, GPRCY4, STRL22
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
sirx6
eFluor 660
Ccr6
Conjugated
Ccr6
IgG2a, kappa
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
12458
Video's

For Research Use Only.