CD215 (IL-15Ra) Rat anti-Mouse, PerCP-eFluor™ 710, Clone: DNT15Ra, eBioscience™
Rat Monoclonal Antibody
Merk: Affymetrix eBioscience 46-7149-80
Overige gegevens : Gewicht : 0.25000kg
BeschrijvingStaining has also been observed with this antibody after pre-incubation of NK cells with exogenous Mouse IL-15/IL-15R Complex, suggesting that DNT15Ra will recognize both Mouse IL-15 Receptor alpha and the Mouse IL-15/IL-15R Complex. Applications Reported: This DNT15Ra antibody has been reported for use in flow cytometric analysis, and intracellular staining followed by flow cytometric analysis. Applications Tested: This DNT15Ra antibody has been tested flow cytometric analysis of mouse bone marrow derived dendritic cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative. Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols. Excitation: 488 nm; Emission: 710 nm;Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered. Interleukin 15 (IL-15RA) is a cytokine receptor that specifically binds IL-15 with high affinity. IL-15 regulates T and natural killer cell activation and proliferation. The IL-15 and IL-2 receptors share two subunits, IL-2R beta and IL-2R gamma, and IL-15RA is structurally related to IL-2RA, an additional IL-2-specific alpha subunit necessary for high affinity IL-2 binding. Unlike IL-2RA, IL-15RA is capable of binding IL-15 with high affinity independent of the other subunits. IL-15RA is thought to enhance cell proliferation and expression of apoptosis inhibitor Bcl-xL and Bcl-2.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|IL-15RA, Interleukin-15 receptor subunit alpha, IL15RA, CD215|
|4° C, store in dark, DO NOT FREEZE!|
For Research Use Only.