CD215 (IL-15Ra) Rat anti-Mouse, PerCP-eFluor™ 710, Clone: DNT15Ra, eBioscience™

Description

Description: This DNT15Ra antibody reacts with the alpha subunit of the Mouse IL-15 Receptor. The IL-15 Receptor alpha subunit is unique to IL-15, while the other two subunits of the receptor, beta and gamma, are shared with IL-2. IL-15R signaling is mediated by Jak/STAT pathways, and results in the activation of neutrophils, dendritic cells, and macrophages. IL-15 has also been found to play a critical role in the development and survival of NK cells and CD8+ T cells. Although high levels of mouse IL-15 mRNA have been observed in many cell types, free IL-15 is rarely detectable in biological samples. Recent research suggests that IL-15 is retained inside the cell and only secreted in complex with IL-15 Receptor alpha. This chaperoning is necessary from the ER to the Golgi, through secretion. The cytokine/receptor complex may stay bound to the cell surface, where it can be trans-presented to cells expressing the beta-gamma subunits of the receptor, or can be secreted in a soluble form lacking the transmembrane domain of IL-15 Receptor alpha. IL-15 and IL-15 Receptor alpha are expressed by dendritic cells and macrophages in response to inflammatory stimuli. Our testing suggests that there may be strain differences in expression and staining of the Mouse IL-15 Receptor alpha. This DNT15Ra antibody has been tested on cells from C57Bl/6 mice.

Staining has also been observed with this antibody after pre-incubation of NK cells with exogenous Mouse IL-15/IL-15R Complex, suggesting that DNT15Ra will recognize both Mouse IL-15 Receptor alpha and the Mouse IL-15/IL-15R Complex. Applications Reported: This DNT15Ra antibody has been reported for use in flow cytometric analysis, and intracellular staining followed by flow cytometric analysis. Applications Tested: This DNT15Ra antibody has been tested flow cytometric analysis of mouse bone marrow derived dendritic cells. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative. Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols. Excitation: 488 nm; Emission: 710 nm;Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered. Interleukin 15 (IL-15RA) is a cytokine receptor that specifically binds IL-15 with high affinity. IL-15 regulates T and natural killer cell activation and proliferation. The IL-15 and IL-2 receptors share two subunits, IL-2R beta and IL-2R gamma, and IL-15RA is structurally related to IL-2RA, an additional IL-2-specific alpha subunit necessary for high affinity IL-2 binding. Unlike IL-2RA, IL-15RA is capable of binding IL-15 with high affinity independent of the other subunits. IL-15RA is thought to enhance cell proliferation and expression of apoptosis inhibitor Bcl-xL and Bcl-2.