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CD227 (Mucin 1), eFluor 615, clone: SM3, eBioscience™

Mouse Monoclonal Antibody

Merk:  Affymetrix eBioscience 42-9893-82

Overige gegevens : Gewicht : 0.25000kg

 Bekijk meerdere types van dit product via de

Produktcode. 15331130

  • € 300.00 / 100 µg


Geschatte verzenddatum
bij leverancier 26-06-2018
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Beschrijving

Beschrijving

Description: This SM3 monoclonal antibody reacts with the under-glycosylated form of human Mucin 1 (MUC1, CD227), a large glycoprotein belonging to the mucin protein family. Mucin 1 contains a polypeptide core consisting of multiple tandem repeats that become highly glycosylated. Mucin 1 is typically expressed in ductal or glandular epithelial cells and is localized to the apical membrane. In cancerous cells, Mucin 1 expression is increased and membrane-specific localization is lost resulting in expression throughout the membrane and cytoplasm. High levels of under-glycosylated Mucin 1 are thought to affect cell behavior during both invasion and metastasis as well as in immune recognition. In addition, under-glycosylated Mucin 1 is shed from the epithelial cell surface and can be detected in circulation. Alterations in Mucin 1 glycosylation are found in most adenocarcinomas of the breast, lung, pancreas, prostate, and ovary. Mucin 1 has recently been shown to co-localize and interact with members of the erbB receptor kinase family, proteins that are upregulated in more aggressive forms of breast cancer.Please note this antibody sees a distinct epitope from other Mucin 1 antibodies.Applications Reported: This SM3 antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded tissue sections (IHC-P) and immunocytochemistry (ICC).Applications Tested: This SM3 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded human breast carcinoma tissue and by immunocytochemistry on fixed MCF7 cells at less than or equal to 20 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. This product has not been validated for flow cytometric analysis.Filter Recommendation: When using this eFluor® 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength (for example, Excitation 560/55, 585LP, Emission 645/75). A standard Alexa Fluor® 594 filter is acceptable.Excitation: 595 nm; Emission: 615 nm.Filtration: 0.2μm post-manufacturing filtered.
Specificatie

Specificatie

CD227 (Mucin-1)
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P15941
Epithelial Membrane Antigen, EMA, CD227, Episialin, CD227, EMA, H23AG, KL-6, MAM6, MUC-1, MUC-1/SEC, MUC-1/X, MUC1/ZD, PEM, PEMT, PUM, DF3 antigen, H23 antigen, breast carcinoma-associated antigen DF3
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Immunohistochemistry
SM3
eFluor 615
MUC1
Conjugated
MUC1
IgG1
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
4582
Video's

For Research Use Only.