Invitrogen™ CD23 Monoclonal Antibody (EBVCS2), PE-Cyanine7, eBioscience™

Description

Description: The EBVCS2 monoclonal antibody reacts with human CD23, a 45 kDa type II transmembrane glycoprotein. CD23 is expressed on mature B cells, mantle zone B cells, follicular dendritic cells and at low levels on T, NK, langerhans cells and platelets. Expression of CD23 is upregulated upon B cell activation, and soluble forms of the antigen have been reported to be biologically active. CD23 is a low affinity receptor for IgE and is thought to play a role in the regulation of IgE response and B cell activation. CD21 and the alpha subunit of CD11b and CD11c bind to CD23. Applications Reported: This EBVCS2 antibody has been reported for use in flow cytometric analysis. Applications Tested: This EBVCS2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation....

CD23 is a 45 kDa glycoprotein that serves as a low-affinity receptor for IgE, playing a crucial role in regulating IgE responses and B cell activation. It is expressed on mature B cells, mantle zone B cells, follicular dendritic cells, and at lower levels on T cells, NK cells, Langerhans cells, and platelets. CD23 expression is upregulated upon B cell activation, and its soluble forms are biologically active, acting as potent mitogenic factors. CD23 is strongly expressed on Epstein-Barr virus (EBV)-transformed B lymphoblasts and is present on a subpopulation of freshly isolated peripheral blood and tonsil B cells. It is also detected in neoplastic cells from cases of B cell chronic lymphocytic leukemia and some centroblastic/centrocytic lymphomas. Functionally, CD23 is involved in B cell growth, differentiation, and IgE production. It interacts with CD21 and the alpha subunits of CD11b and CD11c, further influencing immune responses. Diseases associated with CD23 dysfunction include chronic conjunctivitis and chronic lymphocytic leukemia, highlighting its importance in both normal immune function and disease states. This makes CD23 a valuable target for antibody customers interested in B-cell-related research and diagnostics.