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CD272 (BTLA), APC, clone: 8F4, eBioscience™

Mouse Monoclonal Antibody

Merk:  Affymetrix eBioscience 17-5956-82

Overige gegevens : Gewicht : 0.00001kg

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Produktcode. 15330800

  • € 273.00 / 100 µg


Geschatte verzenddatum
bij leverancier 27-08-2018
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Beschrijving

Beschrijving

Description: The BTLA antibody reacts with mouse BTLA, B and T lymphocyte attenuator from both BALB/c and C57Bl/6 strains. BTLA is expressed by peripheral lymphocytes, splenic macrophages, developing B cells in the bone marrow and developing T cells in thymus and mature, but not immature bone marrow-derived dendritic cells. BTLA is implicated as a negative regulator of the activation and/or function of various hemopoietic cell types. It is reported that BTLA binds to B7-H4, but further studies are needed to confirm this interaction.Note: The anti-mouse BTLA monoclonal antibody 6F7 is reported to stain CD4+ and CD8+ single-positive (SP) thymocytes (Hurchla et al). However, other anti-mouse BTLA clones generated simultaneously with 6F7 (8F4, 3F9.D12, 6G3 and 6H6) do not stain SP thymocytes. It is not understood why there is a discrepancy in thymocyte staining. However, in staining of splenocytes and bone marrow cells, clones 8F4, 3F9.D12, 6G3 and 6H6 stain similar populations to 6F7.Applications Reported: This 8F4 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 8F4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
Specificatie

Specificatie

CD272 (BTLA)
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Q7TSA3
BTLA1, CD272, B and T lymphocyte attenuator, B- and T-lymphocyte attenuator, B- and T-lymphocyte-associated protein
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
8F4
APC
Btla
Conjugated
Btla
IgG1, kappa
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
208154
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