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CD273 (B7-DC) Monoclonal Antibody (TY25), Functional Grade, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

€ 130.00 - € 256.00

Specifications

Antigen CD273 (B7-DC)
Clone TY25
Concentration 1 mg/mL
Applications Flow Cytometry, Functional Assay, Neutralization
Classification Monoclonal
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Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15248727
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Affymetrix eBioscience
16-5986-82
100 μg
€ 256.00
100µg
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Estimated Shipment: 07-05-2024
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15238727
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Affymetrix eBioscience
16-5986-81
50 μg This item is currently unavailable or has been discontinued.
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Description

Description

Description: The TY25 monoclonal antibody reacts with mouse B7-DC/PD-L2. B7-DC, a member of the B7 family, has a predicted molecular weight of ∽25 kDa and belongs to the Ig superfamily. The mouse B7-DC has a short cytoplasmic tail (4aa). B7-DC is primarily expressed by sub-populations of dendritic cells, monocytes and macrophages. Although B7-DC has structural and sequence similarities to the B7 family, it does not bind CD28/CTLA-4, but binds PD-1. The interactions between PD-1 and B7-DC/PD-L2 have been reported to be involved in costimulation or suppression of T cell proliferation depending on state of cellular activation. TY25 is a useful tool to study the exact function of B7-DC/PD-L2 in APC/T cell interaction and to characterize the expression pattern of this molecule in mouse. Applications Reported: The TY25 antibody has been reported for use in flow cytometric analysis. It has also been reported for inhibition of ligand binding. Applications Tested: The TY25 antibody has been tested by flow cytometric analysis of mouse splenocytes and B7-DC transfected cells. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Programmed death-ligand 2 (PD-L2), or B7-DC, is a member of the B7 ligand family within the immunoglobulin superfamily that, along with programmed death-ligand 1 (PD-L1), acts as a ligand for programmed cell death protein 1 (PD-1). Though expressed primarily in dendritic cells, PD-L2 expression can be induced on a wide variety of immune and non-immune cells depending on the microenvironment. PD-L2 expression is particularly upregulated in the presence of Th2 cytokine, IL-4, as well as Th1 cytokines, TNF-alpha and IFN-gamma to a lesser degree. While generally expressed at lower levels compared to PD-L1, PD-L2 demonstrates a 2 to 6 times higher relative affinity to PD-1 than PD-L1. PD-1 and its ligands are referred to as inhibitory immune checkpoint molecules in that they provide useful negative feedback during physiological homeostasis. Ligation of PD-L2 or PD-L1 inhibits activation, proliferation, and cytokine secretion (e.g. IFN-gamma, IL-10) in T cells, μgtimately dampening immune response. Conversely, studies have shown that PD-L2 can also stimulate T cell proliferation and cytokine production, even in PD-1-deficient T cells, suggesting additional receptors. Recent studies have concluded that PD-L2 also binds to a second receptor, repulsive guidance molecule b (RGMb), which was originally identified as a receptor for bone morphogenetic proteins (BMPs). RGMb is expressed in the central nervous system, as well as in macrophages, however, its role in immunity is only beginning to emerge. Interaction between PD-L2 and RGMb regulates the development of respiratory tolerance in the lung through BMP and/or neogenin signaling pathways. The naturally occurring human PD-L2 monomer consists of a 201-amino-acid extracellular domain, a 21-amino-acid transmembrane domain, and a 32-amino-acid cytoplasmic domain.
Specifications

Specifications

CD273 (B7-DC)
1 mg/mL
Monoclonal
Liquid
RUO
PBS with no preservative; pH 7.2
B7 dendritic cell molecule; B7DC; B7-DC; bA574F11.2; Btdc; Butyrophilin B7-DC; butyrophilin-like protein; CD273; F730015O22Rik; MGC124039; MGC124040; PD1 ligand 2; PD-1 ligand 2; PD-1-ligand 2; PDCD1 ligand 2; PDCD1L2; Pdcd1lg2; Pdl2; PD-L2; Programmed cell death 1 ligand 2; programmed death ligand 2
PDCD1LG2
Primary
4° C
PDCD1LG2
TY25
Flow Cytometry, Functional Assay, Neutralization
Functional Grade
Rat
Mouse
Q9WUL5
58205
IgG2a κ
Affinity chromatography
Antibody
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