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CD279 (PD-1), PerCP-eFluor™ 710, clone: RMP1-30, eBioscience™

Rat Monoclonal Antibody

Merk:  Affymetrix eBioscience 46-9981-82

Overige gegevens : Gewicht : 0.25000kg

 Bekijk meerdere types van dit product via de

Produktcode. 15331410

  • € 282.00 / 100 µg


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Beschrijving

Beschrijving

Description: The RMP1-30 antibody reacts with mouse PD-1 (programmed death-1), a 55kDa member of the Ig superfamily. PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease in mice. PD-1 is expressed mainly on activated T and B lymphocytes. Two novel B7 Family members have been identified as PD-1 ligands, PD-L1 (B7-H1) and PD-L2 (B7-DC). Evidence reported to date suggests overlapping functions for these ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. RMP1-30 does not block the binding of either B7-H1-Ig or B7-DC-Ig to PD-1 transfectants.Applications Reported: This RMP1-30 antibody has been reported for use in flow cytometric analysis.Applications Tested: This RMP1-30 antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative. Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specificatie

Specificatie

CD279 (PD-1)
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Q02242
PD1
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
RMP1-30
PerCP-eFluor 710
Pdcd1
Conjugated
Pdcd1
IgG2b, kappa
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
18566
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For Research Use Only.