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CD28, PE-Cyanine7, clone: 37.51, eBioscience™

Syrian Hamster Monoclonal Antibody

Merk:  Affymetrix eBioscience 25-0281-81

Overige gegevens : Gewicht : 0.25000kg

 Bekijk meerdere types van dit product via de

Produktcode. 15360910

  • € 158.00 / 50 µg


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Beschrijving

Beschrijving

Description: The 37.51 monoclonal antibody reacts with the mouse CD28 molecule, a 45kDa homodimer expressed by thymocytes, mature T cells and NK cells. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2) and is a potent costimulator of T cells. Signaling through CD28 augments IL-2 and IL-2 receptor expression as well as cytotoxicity of CD3-activated T cells.Applications Reported: This 37.51 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 37.51 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specificatie

Specificatie

CD28
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P31041
MGC138290, Tp44, CD28 antigen, T-cell-specific surface glycoprotein CD28
Golden Syrian Hamster
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
37.51
PE-Cyanine7
Cd28
Conjugated
Cd28
IgG
50μg
Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling and experimental procedures.
Primary
12487
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