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CD317 (BST2, PDCA-1), eFluor 450, clone: eBio927, eBioscience™

Rat Monoclonal Antibody

Merk:  Affymetrix eBioscience 48-3172-80

Overige gegevens : Gewicht : 0.25000kg

 Bekijk meerdere types van dit product via de

Produktcode. 15361760

  • € 124.00 / 25 µg


onze contactgegevens 020 4877000
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Beschrijving

Beschrijving

Description: The eBio927 monoclonal antibody reacts with PDCA-1 (BST2, CD317), a specific marker of plasmacytoid dendritic cells (pDC), also known as type I IFN-producing cells (IPC) in the naive mouse. Mouse IPCs are typically CD11c+, CD11b-, B220+, Ly-6C+, and CD62L+. PDCA-1 is predominantly expressed by IPCs in the naive mouse which represents a very minor population (<0.5%) of splenocytes. It is upregulated on numerous cell types following stimulation which triggers an IFN response. PDCA-1 cycles between cell surface and intracellular compartments and may function to regulate trafficking of secreted cytokines. PDCA-1 (BST2) is the protein recognized by antibody 120G8. The eBio927 monoclonal antibody has also been shown to have functional activity. The epitope recognized by eBio927 is distinct from eBio129c; thus, the antibodies can be used to costain, purify and identify pDCs.Applications Reported: This eBio927 antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBio927 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor™ 450 is an alternative to Pacific Blue™. eFluor™ 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2μm post-manufacturing filtered.
Specificatie

Specificatie

CD317 (BST2, PDCA-1)
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Q8R2Q8
Bone marrow stromal cell antigen 2, Tetherin, PDCA-1, 2310015I10Rik, Bst-2, C87040, DAMP-1, GREG, DAMP-1 protein homolog, HM1.24 antigen, bone marrow stromal antigen 2
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
eBio927
eFluor 450
Bst2
Liquid
Bst2
IgG2b, kappa, kappa
25μg
4° C, store in dark, DO NOT FREEZE!
Primary
69550
Video's

For Research Use Only.