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CD33 Monoclonal Antibody (P67.6), Super Bright™ 600, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 63-0337-41

104.67 EUR valid until 2024-03-29
Use promo code "21615" to get your promotional price.



Additional Details : Weight : 0.01000kg

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Product Code. 15943494

  • € 157.00 / Pack of 25
Estimated Shipment: 11-04-2024
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Description

Description

Description: The P67.6 monoclonal antibody reacts with human CD33 (also known as GP67 and P67), a 67 kDa type I transmembrane glycoprotein that is a member of the Siglec (sialic acid-binding Ig superfamily lectin) family. It is highly specific to the hematopoietic compartment and is expressed on monocytes, activated T cells, granulocytes, myeloid progenitors, and mast cells.

Applications Reported: This P67.6 antibody has been reported for use in flow cytometric analysis. Applications Tested: This P67.6 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm. We recommend using a 610/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 405 nm; Emission: 600 nm; Laser: Violet Laser Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Specifications

Specifications

CD33
Monoclonal
5 μL/Test
PBS with BSA and 0.09% sodium azide; pH 7.2
P20138
CD33
Affinity chromatography
RUO
945
4° C, store in dark, DO NOT FREEZE!
Liquid
Flow Cytometry
P67.6
Super Bright 600
CD33
CD33; CD33 antigen; CD33 antigen (gp67); CD33 molecule; CD33 molecule transcript; FLJ00391; gp67; Myeloid cell surface antigen CD33; p67; sialic acid binding Ig-like lectin 3; sialic acid-binding Ig-like lectin 3; SIGLEC3; Siglec-3
Mouse
25 Tests
Primary
Human
Antibody
IgG1 κ
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