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CD45R (B220), PerCP-eFluor™ 710, clone: RA3-6B2, eBioscience™

Rat Monoclonal Antibody

Merk:  Affymetrix eBioscience 46-0452-82

Overige gegevens : Gewicht : 0.25000kg

 Bekijk meerdere types van dit product via de

Produktcode. 15331260

  • € 268.00 / 100 µg


Geschatte verzenddatum
bij leverancier 27-08-2018
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Beschrijving

Beschrijving

Description: The RA3-6B2 monoclonal antibody reacts with exon A-restricted isoform of mouse CD45, a 220kDa surface molecule. CD45R/B220 epitope is mainly expressed by the B cell lineage from early Pro-B to mature B cells. However, some activated T cells, lymphokine activated killer cells (LAK), NK cell progenitors in the bone marrow, and T cells of the \I{lpr/lpr\I} mutant mouse also express this antigen.Applications Reported: This RA3-6B2 antibody has been reported for use in flow cytometric analysis.Applications Tested: This RA3-6B2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specificatie

Specificatie

CD45R
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P06800, P08575
B220, CD45R, Cd45, Ly-5, Lyt-4, T200, Cluster designation 45R
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
HumanþMurine
Flow Cytometry
RA3-6B2
PerCP-eFluor 710
PTPRC
Conjugated
Ptprc
IgG2a, kappa
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
5788
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For Research Use Only.