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IBA Lifesciences CD45RO Fab Streptamer™ Isolation Kit MB

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Quantity:
1 Kit
Unit Size:
1 set
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Product Code. 15302086

Brand: IBA Lifesciences 68000209

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Description

CD45RO Fab Streptamer Isolation Kit MB is for reversible cell isolation delivering label-free target cells.

  • Strep-Tactin™ Magnetic Microbeads for Fab Streptamers™; 2 vials for 5 x 108 cells each
  • CD45RO Fab-Strep, human for isolation of T-cells; 5 vials for 2 x 108 cells
  • Biotin stock solution for Streptamer™ technology; 2 x 1 mL
  • Buffer IS, Streptamer™ Washing Buffer, 10x concentrated; 50 mL
  • Microbeads are intended for use with a permanent magnet for manual cell isolation; we recommend our StrepMan magnet for 2 x 15 mL and 2 x 50 mL Falcon tubes.
  • Principle of Selection: Isolation of human CD45RO T cells is performed by positive selection.
  • PBMCs are labeled with magnetic CD45RO Fab Streptamers™.
  • Labeled cells are isolated in a strong magnet (StrepMan, horizontal position) where they migrate toward the tube wall on the side of the magnet.
  • This CD45RO positive cell fraction is collected and cells are liberated from all labeling reagents by addition of biotin in a strong magnet (StrepMan, upright position).
  • The magnetic Streptamers™ migrate toward the tube wall and the label-free cells remain in the supernatant.
  • Biotin is removed by washing.
  • The resulting cell preparation is highly enriched with CD45RO+ T cells with a purity of more than 90%.
  • No depletion steps and no columns are needed.

Specifications

Cell Type PBMCs
Material Magnetic Microspheres Coated with Strep-Tactin
No. of Cells 1 x 109
Quantity 1 Kit
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