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CD62L (L-Selectin) Monoclonal Antibody (MEL-14), PE-Cyanine5, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
€ 138.00 - € 406.00
Specifications
Clone | MEL-14 |
---|---|
Concentration | 0.2 mg/mL |
Applications | Flow Cytometry |
Classification | Monoclonal |
Conjugate | PE-Cyanine5 |
Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
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Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
15340490
|
Affymetrix eBioscience
15-0621-82 |
100 μg |
€ 242.00
100µg |
In Stock Log in to see stock. |
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15350490
|
Affymetrix eBioscience
15-0621-83 |
200 μg |
€ 406.00
200µg |
Estimated Shipment: 29-05-2024 Log in to see stock. |
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15330490
|
Affymetrix eBioscience
15-0621-81 |
50 μg |
This item is currently unavailable or has been discontinued. View the product page for possible alternatives. |
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Description
Description: The MEL-14 monoclonal antibody reacts with mouse CD62L, a 76 kDa member of the selectin family. CD62L is expressed by neutrophils, monocytes, and subsets of T, B, and NK cells and binds a number of glycosylated, fucosylated, sulfated sialylated glycoproteins including CD34, glycam-1 and MAdCam-1. These interactions mediate rolling of lymphocytes on activated endothelium at the sites of inflammation and homing of cells to the high endothelial venules (HEV) of peripheral lymphoid tissues. Applications Reported: The MEL-14 antibody has been reported for use in flow cytometric analysis. Applications Tested: The MEL-14 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat.
The human CD62L is a 74-95 kDa glycoprotein member of the selectin family of adhesion receptors. L-Selectin is comprised of an aminoterminal C-type lectin binding domain, an epidermal growth factor-like domain, two short consensus repeat (SCR) sequences homologous to those found in complement binding proteins, a short spacer region, a transmembrane region and a short cytoplasmic region. Human CD62L (L-Selectin) is constitutively expressed on all classes of leukocytes including lymphocytes (except a substantial population of memory T cells), monocytes and polymorphonuclear cells.Specifications
MEL-14 | |
Flow Cytometry | |
PE-Cyanine5 | |
Rat | |
Mouse | |
20343 | |
Primary | |
4° C, store in dark, DO NOT FREEZE! |
0.2 mg/mL | |
Monoclonal | |
Liquid | |
RUO | |
P18337 | |
IgG2a κ | |
Affinity chromatography | |
Antibody |
For Research Use Only.