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IBA Lifesciences CD8 Fab-TACS™ Agarose Column Starter Kit

Label-free isolation of CD8+ T cells

Brand: IBA Lifesciences 6-3305-002

Product Code. 17287740

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Description

Fab-TACS™ - Traceless Affinity Cell Selection - is a positive cell isolation approach based on our Strep-tag™ technology.
Strep-Tactin™ TACS Agarose columns are filled with Strep-Tactin™ coated agarose macrobeads developed for cell isolation. Fab fragments fused to a Twin-Strep-tag™ (Fab-Streps) bind to the matrix.
Target cells adhere to the matrix based on the exclusive binding of the Fab-Strep to a surface marker. Non-target cells are efficiently washed away.
In a final step, the addition of biotin causes the elution of the target cells and the Fab-Streps.
After elution, the Fab-Streps self-dissociate from the cell surfaces due to their reduced affinity.
The label-free authentic target cells are now ready for further downstream applications

Specifications

Includes	Strep-Tactin™ TACS Agarose columns, 0.3 mL (2 x), CD8 Fab-Strep (mouse), 10 x Buffer CI, Biotin stock solution, TACS Column Adapter
Donor Source	Murine splenocytes
Target Species	Mouse
Buffer	10 x Buffer Cl

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